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Modified Proteins and Methods of Making and Using Same

  • US 20110262903A1
  • Filed: 02/25/2011
  • Published: 10/27/2011
  • Est. Priority Date: 02/26/2010
  • Status: Abandoned Application
First Claim
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1. A method of detecting a nucleotide incorporation, comprising:

  • (a) performing a nucleotide incorporation using a modified polymerase and generating one or more hydrogen ions as a by-product of the nucleotide incorporation, where the modified polymerase includes one or more amino acid substitutions that reduce the buffering capacity of the modified polymerase relative to an unmodified polymerase; and

    (b) detecting the presence of the one or more hydrogen ions generated as a by-product of the nucleotide incorporation, thereby detecting the nucleotide incorporation.

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