METHOD FOR PRETREATING SPECIMEN AND METHOD FOR ASSAYING BIOLOGICAL SUBSTANCE
First Claim
1. A system for assaying a biologically-relevant substance in a specimen, comprising:
- a first support to which a substance having affinity to a contaminant contained in the specimen, a substance that inactivates the contaminant, or a substance having affinity to the biologically-relevant substance in the specimen is immobilized; and
a second support selected from a support to which a reagent for detecting the biologically-relevant substance is immobilized and a support made of a solid-phased reagent for detecting the biologically-relevant substance.
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Accused Products
Abstract
On magnetic particles serving as a first support, an antibody against a nonspecific reaction factor is immobilized. These magnetic particles are mixed with a specimen and suspended therein. After suspending, the suspension is sucked up into a pipette chip and a magnet comes close to the pipette chip. While remotely constraining with the magnet the magnetic particles carrying the nonspecific reaction factor bonded thereto, the residual liquid is discharged into a well. Thus, the removal of a contaminant contained in the specimen is completed. The thus treated specimen discharged into the well is subjected to an immunoassay. The magnetic particles carrying the antibody immobilized thereon are mixed with the treated specimen and suspended therein. The suspension is sucked up into a pipette chip and the magnetic particles carrying an antigen bonded thereto are separated by use of the magnet. The magnetic particles carrying the antigen bonded thereto are washed, mixed with an enzyme-labeling solution, which contains a second support, and suspended therein. After suspending, the magnetic particles being labeled and carrying the antigen bonded thereto are mixed with a substrate solution and subjected to the measurement of emission intensity, etc.
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Citations
45 Claims
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1. A system for assaying a biologically-relevant substance in a specimen, comprising:
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a first support to which a substance having affinity to a contaminant contained in the specimen, a substance that inactivates the contaminant, or a substance having affinity to the biologically-relevant substance in the specimen is immobilized; and a second support selected from a support to which a reagent for detecting the biologically-relevant substance is immobilized and a support made of a solid-phased reagent for detecting the biologically-relevant substance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 33, 34)
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14. A cartridge comprising:
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a first holding portion, in which a first support to which a substance having affinity to a contaminant contained in a specimen, a substance that inactivates the contaminant, or a substance having affinity to the biologically-relevant substance in the specimen is immobilized is held in advance; a second holding portion, in which a second support selected from a support to which a reagent for detecting the biologically-relevant substance is immobilized and a support made of a solid-phased reagent for detecting the biologically-relevant substance is held; and a specimen holding portion in which the specimen is held. - View Dependent Claims (15, 16, 17, 18, 19, 30, 31, 32)
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20. A method for pretreating a specimen before assaying a biologically-relevant substance in the specimen, comprising:
a step of treating the specimen using a first support to which a substance having affinity to a contaminant contained in the specimen, a substance that inactivates the contaminant, or a substance having affinity to the biologically-relevant substance in the specimen is immobilized. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29)
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35. A nucleic acid assay device, comprising:
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(a) a specimen holding portion in which a specimen is held; (b) a first holding portion in which trapping particles for trapping a target nucleic acid from the specimen are held; (c) a second holding portion in which a reagent for detecting the target nucleic acid is held; (d) a dispensing mechanism for dispensing the specimen into the specimen holding portion, a mechanism for mixing the specimen with the trapping particles to extract the target nucleic acid from the specimen, and a mechanism for mixing the extracted target nucleic acid with the reagent for detecting; and (e) a mechanism selected from the group consisting of;
a mechanism for pouring a hydrophobic fluid, which has a specific gravity smaller than that of a mixed fluid of the target nucleic acid and the reagent for detecting, into the second holding portion;
a mechanism for removing or putting lids for covering the respective holding portions;
a mechanism for irradiating an irradiating light for letting the target nucleic acid fluoresce;
a mechanism for receiving a light from the target nucleic acid irradiated with the irradiating light to detect the target nucleic acid; and
a mechanism in which the mechanisms are combined. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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Specification