INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
First Claim
1. An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate, such transistor array having disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid, wherein such transistor array has a pitch of 10 μ
- m or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
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Accused Products
Abstract
The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.
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Citations
32 Claims
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1. An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate, such transistor array having disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid, wherein such transistor array has a pitch of 10 μ
- m or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An integrated sensor array comprising:
a plurality of sensors formed in a circuit supporting substrate, each sensor comprising a chemical field-effect transistor, the sensors being in a planar array of greater than 256 sensors at a density greater than 100 sensors per mm2, each sensor of the array being configured to provide at least one output signal related to a concentration or presence of a chemical or biological sample proximate thereto, such output signal being substantially the same for each sensor of the array in response to the same concentration or presence of the same chemical or biological sample. - View Dependent Claims (14, 15, 23, 24)
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16-22. -22. (canceled)
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25. A single chip chemical assay device comprising:
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a sensor array formed in a circuit supporting substrate, each sensor of the array comprising a chemical field-effect transistor and being configured to provide at least one output signal related to a concentration or presence of a chemical or biological sample proximate thereto, such output signal being substantially the same for each sensor of the array in response to the same concentration or presence of the same chemical or biological sample; a plurality of sample-retaining regions in the circuit supporting substrate, each sample-retaining region disposed on at least one sensor; and control circuitry in the circuit supporting substrate coupled to the sensor array to receive samples of the output signals from said chemical field effect transistors at a rate of at least one frame per second. - View Dependent Claims (26, 31)
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27-30. -30. (canceled)
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32-36. -36. (canceled)
Specification