Stacked-Electrode Peptide-Fragmentation Device

• US 20110266434A1
• Filed: 06/30/2009
• Published: 11/03/2011
• Est. Priority Date: 07/01/2008
• Status: Active Grant

• Current Assignee: Waters Technologies Corporation (Waters Corporation)
• Original Assignee: Waters Technologies Corporation (Waters Corporation)
• Inventors: Jarrell, Joseph A., Li, Guo-Zhong
• Granted Patent: US 8,389,932 B2
• Time in Patent Office: Days
• US Class Current: 250/282
• CPC Class Codes: H01J 49/0072 by ion/ion reaction, e.g. electron transfer dissociation, proton transfer dissociation
• CPC Class Codes: H01J 49/065 having stacked electrodes, e.g. ring stack, plate stack

Abstract

A chemical processing apparatus includes a fragmentation device that includes a linear set of stacked electrodes and a voltage control module that forms DC potential wells of opposite polarity for mutual confinement of opposite polarity ions. A method of protein analysis includes confining positive peptide ions and negatively charged reagent anions in, respectively, first and second DC potential wells in a fragmentation device, mixing the ions, in the fragmentation device, and analyzing ion fragments formed in the mixture.

Claims

1. A method of protein analysis, comprising:

providing a fragmentation device comprising stacked electrodes that each define an aperture, the apertures defining an ion-manipulation region and an axial direction of the fragmentation device;

confining positive peptide ions in a first DC potential well in the ion-manipulation region;

confining negatively charged particles in a second DC potential well in the ion-manipulation region;

reducing a DC barrier between the first and second DC potential wells to mix the positive peptide ions and the negatively charged particles, in the ion-manipulation region, to fragment at least some of the positive peptide ions; and

mass analyzing at least some of the peptide ion fragments.

2. The method of claim 1, further comprising applying at least a first DC voltage to at least two contiguous electrodes of the stacked electrodes to define the first DC potential well, and applying at least a second DC voltage to at least two different contiguous electrodes of the stacked electrodes to define the second DC potential well.

3. The method of claim 1, wherein the negatively charged particles comprise electrons, and further comprising forming a magnetic field to confine the electrons in the ion-manipulation region in a radial direction that is perpendicular to the axial direction.

4. The method of claim 3, wherein the magnetic field has a field direction parallel to the axial direction of the fragmentation device.

5. The method of claim 3, wherein the electrons have a kinetic energy in a range of about 0.02 eV to about 5 eV.

6. The method of claim 1, wherein the electrodes comprise ring electrodes.

7. The method of claim 1, wherein the negatively charged particles comprise reagent anions.

8. The method of claim 1, wherein reducing the DC potential barrier comprises reducing the depth of the second DC potential well.

9. The method of claim 1, wherein the first DC potential well has a depth of about −

10 volts or less.

10. The method of claim 1, wherein the second DC potential well has a depth of about +10 volts or less.

11. The method of claim 1, further comprising confining additional positive peptide ions in a third DC potential well in the ion-manipulation region.

12. The method of claim 11, wherein the second DC potential well is disposed between the first and third potential wells.

13. A chemical processing apparatus, comprising:

a fragmentation device, comprising a plurality of electrodes disposed in series along a longitudinal axis of the fragmentation device and defining an ion-manipulation region;

means for applying at least a first DC voltage to at least two contiguous electrodes of the plurality of electrodes to define a first DC potential well, and applying at least a second DC voltage to at least two different contiguous electrodes of the plurality of electrodes to define a second DC potential well;

means for reducing a DC potential barrier between the first and second DC potential wells to permit positive peptide ions, confined in the first DC potential well, and negatively charged particles, confined in the second DC potential well, to mix; and

a mass-spectrometry module for analyzing at least some fragments of the positive peptide ions extracted from the mixture.

14. The mass spectrometer of claim 13, further comprising a control unit comprising the means for applying the DC voltages and the means for reducing the DC potential barrier.

15. The mass spectrometer of claim 13, further comprising means for introducing a magnetic field in the fragmentation device for radial containment of the negatively charged particles in the ion-manipulation region.

16. The mass spectrometer of claim 13, wherein the fragmentation device comprises a stacked-ring ion guide.

17. The mass spectrometer of claim 13, wherein the negatively charged particles are electrons, and further comprising a magnetic field generator configured to apply a magnetic field parallel to the longitudinal axis, and an electron source for filling the second DC potential well with electrons having a kinetic energy in a range of about 0.02 eV to about 5 eV.