METHOD FOR DETECTING METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) STRAINS
First Claim
Patent Images
1. A method for detecting MRSA in a sample, comprising:
- per variant A,a) isolating chromosomal DNA of S. aureus from the sample using a genome probe, andb) determining the presence or absence of a nucleotide sequence specific for MRSA in the chromosomal DNA isolated in step a), wherein the presence of the nucleotide sequence indicates the presence of MRSA in the sample;
orper variant B,a) isolating DNA from the sample using a genome probe specific for an MRSA nucleotide sequence, andb) determining the presence or absence of a specific sequence of S. aureus in the DNA isolated in step a) wherein the presence of the specific sequence of S. aureus indicates the presence of MRSA in the sample.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates, inter alia, to a method for detecting methicillin-resistant Staphylococcus aureus (MRSA) strains, wherein one of the following method variants is carried out:
- variant A
- a) chromosomal DNA of S. aureus is isolated from the sample by means of a genome probe, and
- b) a nucleotide sequence which is specific for MRSA is detected in the isolated DNA;
- or
- variant B
- a) DNA is isolated from the sample, wherein the isolation makes use of a genome probe which is specific for an MRSA nucleotide sequence, preferably an MRSA resistance gene, and
- b) the DNA isolated in step a) is tested for specific sequences of S. aureus.
In addition, suitable kits for carrying out the corresponding methods are provided.
11 Citations
15 Claims
-
1. A method for detecting MRSA in a sample, comprising:
-
per variant A, a) isolating chromosomal DNA of S. aureus from the sample using a genome probe, and b) determining the presence or absence of a nucleotide sequence specific for MRSA in the chromosomal DNA isolated in step a), wherein the presence of the nucleotide sequence indicates the presence of MRSA in the sample; or per variant B, a) isolating DNA from the sample using a genome probe specific for an MRSA nucleotide sequence, and b) determining the presence or absence of a specific sequence of S. aureus in the DNA isolated in step a) wherein the presence of the specific sequence of S. aureus indicates the presence of MRSA in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A kit for detecting MRSA in a sample, comprising:
-
per variant A, a) at least one genome probe suitable for isolating DNA of S. aureus, and b) means for detecting a nucleotide sequence specific for MRSA; or per variant B, a) at least one genome probe suitable for isolating from a sample DNA comprising an MRSA resistance gene, and b) means for detecting an S. aureus-specific DNA sequences. - View Dependent Claims (13, 14, 15)
-
Specification