PRODUCTION OF VERY LONG CHAIN POLYUNSATURATED FATTY ACIDS IN OILSEED PLANTS

• US 20110269983A1
• Filed: 03/10/2011
• Published: 11/03/2011
• Est. Priority Date: 02/12/2003
• Status: Abandoned Application

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: CAHOON, EDGAR BENJAMIN, KINNEY, ANTHONY J., LIU, ZHAN-BIN, DAMUDE, HOWARD GLENN
• Application Number:
  US13/044,984
• Publication Number:
  US 20110269983A1
• Time in Patent Office: Days
• US Class Current: 554/230
• CPC Class Codes: A21D 2/165 Triglycerides
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 50/40 for carnivorous animals, e.g. cats or dogs
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 11/01 Pulses or legumes in form of whole pieces or fragments thereof, without mashing or comminuting A23L11/10 – A23L11/70 take precedence
• CPC Class Codes: A23L 11/03 Soya beans, e.g. full-fat soya bean flakes or grits
• CPC Class Codes: A23L 11/05 Mashed or comminuted pulses or legumes; Products made therefrom A23L11/30 takes precedence; tofu or soya cheese A23L11/45; soy drinks A23L11/65
• CPC Class Codes: A23L 11/07 Soya beans, e.g. oil-extracted soya bean flakes A23L11/30 takes precedence
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/1876 Long-chain fatty acids
• CPC Class Codes: A23V 2250/1882 Polyunsaturated fatty acids
• CPC Class Codes: A23V 2300/21 Genetic modification
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: Y02A 40/818 Alternative feeds for fish, e.g. in aquacultures

Abstract

Oilseed plants which have been transformed to produce very long chain polyunsaturated fatty acids, recombinant constructs used in such transformations, methods for producing such fatty acids in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

Claims

1. A transgenic oilseed plant that produces mature seeds in which the total seed fatty acid profile comprises at least 1.0% of at least one omega-3 polyunsaturated fatty acid selected from the group consisting of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA)

wherein said transgenic oilseed plant comprises in its genome at least three transgenic nucleic acid sequences encoding at least three different polypeptides, wherein at least two polypeptides have desaturase activity and at least one polypeptide has elongase activity, and further wherein substrates for desaturase activity and for elongase activity are produced by said transgenic oilseed plant.

16. Seeds obtained from the transgenic oilseed plant of claim 1 wherein said seeds comprise the transgenes.

21. Oil obtained from the seeds of the transgenic oilseed plants of claim 1.

26. The transgenic oilseed plant of claim 1 wherein the transgenic oilseed plant is selected from the group consisting of soybean, Brassica species, sunflower, maize, cotton, flax, and safflower.

134. The transgenic oilseed plant of claim 1 wherein the ratio of EPA:

DHA is in the range from 1;

100 to 860;

100.

136. The transgenic oilseed plant of claim 1 wherein the ratio of DHA:

EPA is in the range from 1;

100 to 110;

100.

138. Seeds obtained from the transgenic oilseed plant of claim 134 or claim 136.

139. Oil obtained from the seeds of the transgenic oilseed plants of claim 134 or claim 136.

2-15. -15. (canceled)

17-20. -20. (canceled)

22-25. -25. (canceled)

27-133. -133. (canceled)

135. (canceled)

137. (canceled)