Electrochemical Biosensor Structure and Measuring Method Using the Same
First Claim
1. An electrochemical biosensor electrode structure comprising:
- a working electrode and a reference electrode used as electrodes for sample measurement being arranged separately from each other in lengthwise direction of a sample insertion path,the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and
at least two sample recognition electrodes used as electrodes for sample recognition being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode.
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Abstract
The present invention provides there is provided an electrochemical biosensor electrode structure that includes: a working electrode and a reference electrode used as electrodes for sample measurement being arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and at least two sample recognition electrodes used as electrodes for sample recognition being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode. The present invention minimizes the effect of the double layer capacitance and independently applies the sample recognition signal to accurately detect the sample insert time and velocity.
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Citations
20 Claims
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1. An electrochemical biosensor electrode structure comprising:
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a working electrode and a reference electrode used as electrodes for sample measurement being arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and at least two sample recognition electrodes used as electrodes for sample recognition being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode. - View Dependent Claims (2, 3, 4, 5)
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6. A structure for electrochemical biosensor comprising:
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a bottom plate comprising electrodes for sample measurement, and at least two sample recognition electrodes arranged separately from the electrodes for sample measurement on an insulating substrate, the electrodes for sample measurement including a working electrode and a reference electrode, the working electrode and the reference electrode being arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode, the at least two sample recognition electrodes being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode; a middle plate having a double-coated adhesive insulating film structure with the sample insertion path formed thereon; and a top plate having at least two air exhaust holes being formed on the insulating substrate in correspondence to the end of the sample insertion path, p1 the at least two air exhaust holes being provided correspondingly to the ends of the at least two sample recognition electrodes to vertically overlap the ends of the at least two sample recognition electrodes. - View Dependent Claims (7, 8, 9)
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10. A sample measurement method using a biosensor, comprising:
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(a) preparing the biosensor having electrodes for sample measurement, and at least two sample recognition electrodes provided separately from the electrodes for sample measurement, the electrodes for sample measurement including a working electrode and a reference electrode, the at least two sample recognition electrodes receiving a sample recognition signal controlled separately from a sample measurement signal applied to the working electrode and the reference electrode; (b) applying the sample recognition signal to the one of the at least two sample recognition electrodes to determine whether a sample has arrived or not, the sample recognition signal being generated and controlled independent of the sample measurement signal; and (c) if the sample has arrived, applying the sample measurement signal to the working electrode and the reference electrode and measuring a response signal to the sample. - View Dependent Claims (11, 12)
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13. A method for fabricating an electrochemical biosensor electrode, comprising:
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forming a first conductive thin film adhesively on an insulating substrate by lamination; coating a photosensitivity dry film on the first conductive thin film by lamination, and performing a photo and etching process to form an electrode pattern comprising a material of the first conductive thin film, the electrode pattern integrally having at least two sample recognition electrodes and electrodes for sample measurement, the electrodes for sample measurement including a working electrode and a reference electrode, the working electrode and the reference electrode being arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and forming a second conductive thin film on the surface of the electrode pattern, the second conductive thin film having a higher conductivity than the electrode pattern. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification