New Molecularly Imprinted Polymer and Method for its Production

• US 20110275540A1
• Filed: 01/22/2010
• Published: 11/10/2011
• Est. Priority Date: 01/22/2009
• Status: Abandoned Application

• Current Assignee: Sten Ohlson
• Original Assignee: Sten Ohlson
• Inventors: Ohlson, Sten
• Application Number:
  US13/145,460
• Publication Number:
  US 20110275540A1
• Time in Patent Office: Days
• US Class Current: 506/15
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/205 Aptamer

Abstract

The present invention relates to a molecularly imprinted polymer and a method of producing the same using complementary oligo- and/or polynucleotides.

Claims

1. A method of producing molecularly imprinted polymers, the method comprising the following steps:

selecting a template molecule,

providing at least two starter oligonucleotides each linked to a functional group and wherein said at least two starter oligonucleotides being the same or different,

bringing the template molecule in contact with the functional groups each linked to said starter oligonucleotides allowing the functional groups to bind to the template molecule,

adding a single stranded oligo- or polynucleotide library allowing the oligo- or polynucleotides of the library showing complementarity to the starter oligonucleotides linked to the functional groups to hybridize in order to form a complex between said at least two starter oligonucleotides and a oligo- or polynucleotide from the library,

forming a complementary strand by a polymerizing reaction wherein the starter oligonucleotides linked to the functional groups serve as primers and thereby forming a nucleotide based double-stranded polymer, and

removing the template molecule leaving the functional groups in spatial and binding complementarity towards the accessible binding sites of the template molecule.

2. The method according to claim 1, wherein one of said at least two starter oligonucleotides has a functional group attached to its 3′

-end and the other starter oligonucleotide has a functional group attached to its 5′

-end.

3. The method according to claim 1, comprising a step of amplifying the nucleotide based double stranded polymers before or after removing the template.

4. The method according to claim 1, wherein the functional groups are selected from the group consisting of amino acids, carbohydrates (mono and oligosaccharides), nucleotides or oligonucleotides, peptides and polypeptides, polyelectrolytes, carboxylic acids, amines, amides, sulphoderivatives such as sulfonamide, phosphoderivatives such as phosphoamide, hydroxyls, aldehydes, ketones, ethers, nitriles, aromatic- and alkyl hydrocarbons, thiols, boronates and imides.

5. The method according to claim 1, wherein the template molecule immobilized to a soluble or insoluble support.

6. The method according to claim 1, wherein the functional groups are linked to the starter oligonucleotides with an inert linker.

7. The method according to claim 1, wherein the nucleotides of the starter oligonucleotides, attached to the functional groups, comprise ribose and/or deoxyribose.

8. The method according to claim 7, wherein the starter oligonucleotides attached to the functional groups comprises about 1-100 nucleotides.

9. The method according to claim 1, further comprising the step of identifying the functional groups by sequencing the corresponding linked oligonucleotide.

10. A molecularly imprinted polymer characterized in that said molecularly imprinted polymer comprises functional groups that are in spatial and binding complementarity towards binding sites of a template molecule corresponding to the template molecule that was used when the molecularly imprinted polymer was produced, and said functional groups are connected by polymerized nucleotides.

11. The molecularly imprinted polymer according to claim 10, wherein the functional groups are able to bind covalently or non-covalently to a template molecule.

12. The molecularly imprinted polymer according to claim 10, wherein the functional groups are selected from the group consisting of amino acids, carbohydrates (mono and oligosaccharides), nucleotides or oligonucleotides, peptides and polypeptides, polyelectrolytes, carboxylic acids, amines, amides, sulphoderivatives such as sulfonamide, phosphoderivatives such as phosphoamide, hydroxyls, aldehydes, ketones, ethers, nitriles, aromatic- and alkyl hydrocarbons, thiols, boronates and imides.

13. The molecularly imprinted polymer according to claim 10, wherein the functional group mediates a metal chelate coordinated binding to the template molecule.

14. The molecularly imprinted polymer according to claim 10, wherein the functional groups are joined to the polymerized nucleotides with an inert linker.

15. The molecularly imprinted polymer according to claim 10, wherein the imprint is composed of single-stranded or double-stranded nucleotide chains.

16. The molecularly imprinted polymer according to claim 10, wherein said the imprinted polymer is soluble in aqueous compositions.