Targeted genomic modification with partially single-stranded donor molecules
First Claim
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1. A method of integrating a linear nucleic acid molecule comprising a double-stranded sequence of interest having first and second ends and a first single-stranded nucleotide at the first end of the double-stranded sequence into the genome of a cell, the method comprising:
- creating a double-stranded break using a nuclease comprising a TAL-effector domain in the genome of the cell such that the linear nucleic acid molecule is integrated at the site of the double-stranded break.
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Abstract
Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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9 Claims
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1. A method of integrating a linear nucleic acid molecule comprising a double-stranded sequence of interest having first and second ends and a first single-stranded nucleotide at the first end of the double-stranded sequence into the genome of a cell, the method comprising:
creating a double-stranded break using a nuclease comprising a TAL-effector domain in the genome of the cell such that the linear nucleic acid molecule is integrated at the site of the double-stranded break. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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