Seeding Cells on Porous Supports

• US 20110294214A1
• Filed: 08/11/2011
• Published: 12/01/2011
• Est. Priority Date: 12/17/2004
• Status: Active Grant

• Current Assignee: Alireza Rezania, Ragae Ghabrial
• Original Assignee: Alireza Rezania, Ragae Ghabrial
• Inventors: Rezania, Alireza, Ghabrial, Ragae
• Granted Patent: US 8,778,673 B2
• Time in Patent Office: Days
• US Class Current: 435/398
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability.

Claims

1. A method for seeding a plurality of cells onto a support comprising the steps of:

a. Introducing a liquid to the support, and

b. Partially removing the liquid from the support, and

c. Contacting the support with a plurality of cells.

2. The method of claim 1, wherein the liquid is media.

3. The method of claim 1, wherein the plurality of cells is a suspension.

4. The method of claim 3, wherein the suspension contains media.

5. The method of claim 1, wherein the support is porous.

6. The method of claim 5, wherein the pores are between about 50 and about 1000 microns.

7. The method of claim 1, wherein the support is hydrophobic.

8. The method of claim 1, wherein the liquid is removed from the support by application of a force.

9. The method of claim 8, wherein the force is centrifugal force.

10. The method of claim 1, wherein the liquid that is removed has a volume, and the plurality of cells is less than or equal to the volume of liquid that is removed.

11. The method of claim 3, wherein the liquid that is removed has a volume, and the plurality of cells is less than or equal to the volume of liquid that is removed.

12. The method of claim 1, wherein the plurality of cells are selected from the group consisting of stem cells, pancreatic precursor/progenitor cells, genetically engineered insulin producing cells, primary pancreatic islets, hepatocytes, chromaffin cells, neural precursor cells, progenitor cells, precursor cells, stem cells, bone marrow cells, umbilical cord blood cells, angioblasts, endothelial cells, osteoblasts, smooth muscle cells, kidney cells, fibroblasts, myofibroblasts, cardiovascular cells, neural cells, neural precursor cells, amniotic cells and post-partum placental cells.

13. A kit for seeding a plurality of cells onto a support comprising a support, media and cells in media.

14. The kit of claim 13, wherein the support is hydrophobic.

15. The kit of claim 13, wherein the matrix of support is made of fibers, comprising material selected from the group consisting of VICRYL, PDS II, PANACRYL, ETHISORB, or any blend thereof.

16. The kit of claim 15, wherein the fibers are incorporated with a foam component.

17. The kit of claim 13, wherein the support is porous.

18. The kit of claim 17, wherein the pores are between about 50 and 1000 microns.

19. The kit of claim 13, wherein the plurality of cells are selected from the group consisting of stem cells, pancreatic precursor/progenitor cells, genetically engineered insulin producing cells, primary pancreatic islets, hepatocytes, chromaffin cells, neural precursor cells, progenitor cells, precursor cells, stem cells, bone marrow cells, umbilical cord blood cells, angioblasts, endothelial cells, osteoblasts, smooth muscle cells, kidney cells, fibroblasts, myofibroblasts, cardiovascular cells, neural cells, neural precursor cells, amniotic cells and post-partum placental cells