NUCLEIC ACID ISOLATION IN PRESERVED WHOLE BLOOD
First Claim
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1. A method of isolating DNA comprising:
- providing a tube containing an anticoagulant agent and a fixative agent selected from the group consisting of diazolidinyl urea, imidazolidinyl urea and combinations thereof;
suspending a sample in the fixative agent;
contacting the sample with an erythrocyte lysis buffer;
contacting the sample with a nucleus lysis buffer; and
contacting the sample with proteinase K and alcohol.
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Abstract
A method for isolating nucleic acids is disclosed, wherein a sample having nucleic acid containing starting material is fixed, lysed, and treated to remove unwanted contaminants. The initial fixing of the sample aids in maintaining the structure and integrity of the isolated DNA and reduces the incidence of end product contaminants and DNA shearing.
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Citations
30 Claims
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1. A method of isolating DNA comprising:
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providing a tube containing an anticoagulant agent and a fixative agent selected from the group consisting of diazolidinyl urea, imidazolidinyl urea and combinations thereof; suspending a sample in the fixative agent; contacting the sample with an erythrocyte lysis buffer; contacting the sample with a nucleus lysis buffer; and contacting the sample with proteinase K and alcohol. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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2-4. -4. (canceled)
Specification