RSV F PROTEIN COMPOSITIONS AND METHODS FOR MAKING SAME
First Claim
Patent Images
1. An immunogenic composition comprising one or more respiratory syncytial virus F (RSV F) polypeptides in which amino acids 100-150 are replaced with the amino acid sequence of SEQ ID NO:
- 9, SEQ ID NO;
12, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
91 or SEQ ID NO;
92.
4 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to immunogenic compositions comprising RSV F protein, methods for preparing compositions that contain RSV F protein ecto-domain polypeptides, and to certain engineered RSV F proteins and nucleic acids that encode the engineered RSV F proteins. Compositions prepared using the methods can contain RSV F protein ecto-domain polypeptides in a predominant or single desired form and conformation. The invention also relates to methods for inducing an immune response to RSV F.
54 Citations
30 Claims
-
1. An immunogenic composition comprising one or more respiratory syncytial virus F (RSV F) polypeptides in which amino acids 100-150 are replaced with the amino acid sequence of SEQ ID NO:
- 9, SEQ ID NO;
12, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
91 or SEQ ID NO;
92. - View Dependent Claims (2, 3, 4, 5, 6, 8, 9, 18)
- 9, SEQ ID NO;
-
7. An immunogenic composition comprising a polypeptide selected from the group consisting of SEQ ID NO:
- 49, SEQ ID NO;
68, SEQ ID NO;
71, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
28, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35, SEQ ID NO;
37, SEQ ID NO;
41, SEQ ID NO;
42, SEQ ID NO;
43, SEQ ID NO;
44, SEQ ID NO;
45, SEQ ID NO;
46, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
51, SEQ ID NO;
52, SEQ ID NO;
53, SEQ ID NO;
54, SEQ ID NO;
55, SEQ ID NO;
57, SEQ ID NO;
58, SEQ ID NO;
59, SEQ ID NO;
60, SEQ ID NO;
61, SEQ ID NO;
62, SEQ ID NO;
63, SEQ ID NO;
64, SEQ ID NO;
65, SEQ ID NO;
66, SEQ ID NO;
67, SEQ ID NO;
69, SEQ ID NO;
70, SEQ ID NO;
85, SEQ ID NO;
86, SEQ ID NO;
87, SEQ ID NO;
88, SEQ ID NO;
89, SEQ ID NO;
93 any of the foregoing sequences in which the signal peptide and/or HIS tag is omitted, and combinations thereof.
- 49, SEQ ID NO;
-
10. A recombinant respiratory syncytial virus F polypeptide (RSV F) in which amino acids 100-150 are replaced with the amino acid sequence of SEQ ID NO:
- 9, SEQ ID NO;
12, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
91 or SEQ ID NO;
92. - View Dependent Claims (11, 12, 13, 15, 16, 17)
- 9, SEQ ID NO;
-
14. A recombinant polypeptide selected from the group consisting of SEQ ID NO:
- 49, SEQ ID NO;
68, SEQ ID NO;
71, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
28, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35, SEQ ID NO;
37, SEQ ID NO;
41, SEQ ID NO;
42, SEQ ID NO;
43, SEQ ID NO;
44, SEQ ID NO;
45, SEQ ID NO;
46, SEQ ID NO;
47, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
51, SEQ ID NO;
52, SEQ ID NO;
53, SEQ ID NO;
54, SEQ ID NO;
55, SEQ ID NO;
57, SEQ ID NO;
58, SEQ ID NO;
59, SEQ ID NO;
60, SEQ ID NO;
61, SEQ ID NO;
62, SEQ ID NO;
63, SEQ ID NO;
64, SEQ ID NO;
65, SEQ ID NO;
66, SEQ ID NO;
67, SEQ ID NO;
69, SEQ ID NO;
70, SEQ ID NO;
85, SEQ ID NO;
86, SEQ ID NO;
87, SEQ ID NO;
88, SEQ ID NO;
89, SEQ ID NO;
93, any of the foregoing sequences in which the signal peptide and/or HIS tag is omitted, and combinations thereof.
- 49, SEQ ID NO;
-
19. A method for producing a composition comprising cleaved RSV F protein ecto-domain polypeptides, the method comprising:
-
a) providing uncleaved RSV F protein ecto-domain polypeptides that contain a protease cleavage site that when cleaved produces F1 and F2 subunits, the amino acid sequence of said uncleaved soluble RSV F protein ecto-domain polypeptides comprising altered furin cleavage sites at positions 106-109 and 133-136, and said uncleaved soluble RSV F protein ecto-domain polypeptides are obtained from a cell that produces them uncleaved; and b) cleaving the provided soluble RSV F protein ecto-domain polypeptides with a protease that cleaves said protease cleavage site, thereby producing a composition comprising cleaved soluble RSV F protein ecto-domain polypeptides. - View Dependent Claims (20)
-
-
21. A method for producing a composition comprising uncleaved RSV F protein ecto-domain polypeptide monomers, trimers or a combination of monomers and trimers, the method comprising:
-
a) providing a biological material that contains uncleaved RSV F protein ecto-domain polypeptides, the amino acid sequence of said uncleaved soluble RSV F protein ecto-domain polypeptides comprising altered furin cleavage sites at positions 106-109 and 133-136, and said uncleaved soluble RSV F protein ecto-domain polypeptides are secreted from a cell that produces them uncleaved; and b) purifying uncleaved RSV F protein ecto-domain polypeptide monomers or trimers from the biological material, thereby producing the composition. - View Dependent Claims (22)
-
-
23. A method for producing a composition comprising uncleaved RSV F protein ecto-domain polypeptide monomers, trimers or a combination of monomers and trimers, the method comprising:
-
a) providing a biological material that contains uncleaved RSV F protein ecto-domain polypeptides, wherein the amino acid sequence of the uncleaved RSV F protein ecto-domain polypeptide contains altered furin cleavage sites, lysine and arginine residues present between about position 101 and about position 161 are deleted or replaced with an amino acid that is not lysine or arginine, the RSV F protein ecto-domain polypeptides are obtained from a host cell that produces them uncleaved between about position 101 and about position 161, and the RSV F protein ecto-domain polypeptides are not cleaved between about position 101 and about position 161; and b) purifying uncleaved RSV F protein ecto-domain polypeptide monomers, trimers or a combination of monomers and trimers from the biological material. - View Dependent Claims (24)
-
-
25. A method for producing a composition comprising cleaved RSV F protein ecto-domain polypeptide monomers, trimers or a combination of monomers and trimers, the method comprising:
-
a) providing biological material that contains cleaved RSV F protein ecto-domain polypeptides that contain an altered fusion peptide; and b) purifying cleaved RSV F protein ecto-domain polypeptides from the biological material. - View Dependent Claims (26)
-
-
27. A method for producing a composition comprising RSV F protein ecto-domain polypeptides, the method comprising:
-
a) providing RSV F protein ecto-domain polypeptides that comprises an altered furin cleavage site at positions 133-136, and said soluble RSV F protein ecto-domain polypeptides are secreted from a cell that produces them in the form of an F2 fragment that is associated with a subunit that comprises F1 but is uncleaved at position 136/137; and b) cleaving the provided RSV F protein ecto-domain polypeptides with a protease that cleaves RSV F protein ecto-domain at a site between positions 101 and 161, thereby producing said composition. - View Dependent Claims (28)
-
-
29. A method for producing a composition comprising RSV F protein ecto-domain polypeptides, the method comprising:
-
a) providing biological material that contains RSV F protein ecto-domain polypeptides that comprise an altered furin cleavage site at positions 133-136, and said soluble RSV F protein ecto-domain polypeptides are secreted from a cell that produces them in the form of an F2 fragment that is associated with a subunit that comprises F1 but is uncleaved at position 136/137, with the proviso that the altered furin cleavage site is not deletion of amino acids 131-134; and b) purifying the RSV F protein ecto-domain polypeptides from the biological material, thereby producing the composition. - View Dependent Claims (30)
-
Specification