Electrochemical Sensor and Method for Manufacturing Same

• US 20110308944A1
• Filed: 02/04/2010
• Published: 12/22/2011
• Est. Priority Date: 02/09/2009
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Fujinawa, Yoshiaki, Tsukada, Masashi, Murase, Yosuke
• Granted Patent: US 8,858,768 B2
• Time in Patent Office: Days
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 2333/902 Oxidoreductases (1.)

Abstract

The present invention provides an electrochemical sensor comprising a substrate, an electrically conductive layer made of carbon particles which is disposed on the substrate, a cell membrane mimetic structure layer containing an enzyme at least one of inside the cell membrane mimetic structure layer and on the interface of the cell membrane mimetic structure layer which is disposed on the electrically conductive layer.

Claims

1. An electrochemical sensor comprising

a substrate,

an electrically conductive layer made of carbon particles which is disposed on the substrate,

a cell membrane mimetic structure layer containing an enzyme at least one of inside the cell membrane mimetic structure layer and on the interface of the cell membrane mimetic structure layer which is disposed on the electrically conductive layer.

2. The electrochemical sensor according to claim 1, wherein the cell membrane mimetic structure layer is a phospholipid membrane.

3. The electrochemical sensor according to claim 2, wherein the phospholipid membrane is a 2-methacryloyloxyethyl phosphorylcholine polymer.

4. The electrochemical sensor according to claim 1, wherein the enzyme is a glucose dehydrogenase.

5. The electrochemical sensor according to claim 1, wherein the enzyme is immobilized on the cell membrane mimetic structure layer in such a state that the enzyme is self-organized with respect to the cell membrane mimetic structure layer.

6. The electrochemical sensor according to claim 1, wherein the carbon particles have a primary particle size of 100 nm or smaller and a specific surface area of 200 m²/g or larger.

7. The electrochemical sensor according to claim 1, wherein the carbon particles are Ketjen Black (trademark) or BLACK PEARLS (trademark).

8. The electrochemical sensor according to claim 1, wherein the electrically conductive layer is formed by screen printing the carbon particles.

9. The electrochemical sensor according to claim 1, wherein the electrically conductive layer is formed by filling the carbon particles.

10. A method for manufacturing an electrochemical sensor comprising

disposing on a substrate an electrically conductive layer made of carbon particles,

disposing on the electrically conductive layer a cell membrane mimetic structure layer, and

immobilizing an enzyme on the cell membrane mimetic structure layer.

11. The method according to claim 10, wherein the cell membrane mimetic structure layer is a phospholipid membrane.

12. The method according to claim 11, wherein the phospholipid membrane is 2-methacryloyloxyethyl phosphorylcholine polymer.

13. The method according to claim 10, wherein the enzyme is a glucose dehydrogenase.

14. The method according to claim 10, wherein the enzyme is immobilized on the cell membrane mimetic structure layer in such a state that the enzyme is self-organized with respect to the cell membrane mimetic structure layer.

15. The method according to claim 10, wherein the carbon particles have a primary particle size of 100 nm or smaller and a specific surface area of 200 m²/g or larger.

16. The method according to claim 10, wherein the carbon particles are Ketjen Black (trademark) or BLACK PEARLS (trademark).

17. The method according to claim 10, wherein the electrically conductive layer is formed by screen printing the carbon particles.

18. The method according to claim 10, wherein the electrically conductive layer is formed by filling the carbon particles.