DETECTION CONJUGATE AND METHOD FOR ANALYSIS
First Claim
1. A detection conjugate for detection of analytes in samples, comprisinga first reagent having a detection conjugate having a binding portion specific for an analyte, a fluorochrome portion connected to the binding portion, and an oligonucleotide linker connected by its first end to the fluorochrome portion, which oligonucleotide linker at least in a section is an oligonucleotide having a quencher section within its nucleic acid sequence, anda second reagent having a quencher that is connected to that end of an oligonucleotide hybridizeable to the quencher section which upon hybridizing with the oligonucleotide linker is located at the first end thereof.
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Accused Products
Abstract
The invention provides a combination of reagents as a test kit containing a detection conjugate having a binding portion and a reagent for deactivating of the fluorochrome portion by interaction with the linker. The binding portion especially is an antibody portion. The detection conjugate has a fluorochrome portion connected to the antibody portion, and a linker connected to the fluorochrome portion, wherein the linker comprises an oligonucleotide. The fluorochrome portion can be deactivated by hydrolysis of the linker or by specific hybridization of a quencher having an oligonucleotide to the linker.
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Citations
25 Claims
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1. A detection conjugate for detection of analytes in samples, comprising
a first reagent having a detection conjugate having a binding portion specific for an analyte, a fluorochrome portion connected to the binding portion, and an oligonucleotide linker connected by its first end to the fluorochrome portion, which oligonucleotide linker at least in a section is an oligonucleotide having a quencher section within its nucleic acid sequence, and a second reagent having a quencher that is connected to that end of an oligonucleotide hybridizeable to the quencher section which upon hybridizing with the oligonucleotide linker is located at the first end thereof.
Specification