MICROCHIP FOR ISOTHERMAL NUCLEIC-ACID AMPLIFICATION REACTION, METHOD FOR PRODUCING THE SAME, AND ISOTHERMAL NUCLEIC-ACID AMPLIFICATION METHOD

• US 20110312036A1
• Filed: 06/13/2011
• Published: 12/22/2011
• Est. Priority Date: 06/22/2010
• Status: Abandoned Application

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Kojima, Kensuke, Yotoriyama, Tasuku, Nakamura, Tomohiko, Watanabe, Hidetoshi, Segawa, Yuji
• Application Number:
  US13/158,935
• Publication Number:
  US 20110312036A1
• Time in Patent Office: Days
• US Class Current: 435/91.2
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 2547/107 Use of permeable barriers, e.g. waxes
• CPC Class Codes: C12Q 2549/101 Hot start
• CPC Class Codes: C12Q 2565/629 being a microfluidic device

Abstract

A microchip for an isothermal nucleic-acid amplification reaction in which at least one of substances necessary for an isothermal amplification reaction of a nucleic acid is present in a reaction region functioning as a reaction field of the reaction, the at least one of the substances being covered with a thin film that melts at a temperature higher than room temperature and lower than a reaction temperature of the reaction.

Claims

1. A microchip for an isothermal nucleic-acid amplification reaction wherein at least one of substances necessary for an isothermal amplification reaction of a nucleic acid is present in a reaction region functioning as a reaction field of the reaction, the at least one of the substances being covered with a thin film that melts at a temperature higher than room temperature and lower than a reaction temperature of the reaction.

2. The microchip according to claim 1, wherein at least one of the remaining substances necessary for the reaction is fixed on the thin film covering the at least one of the substances necessary for the reaction.

3. The microchip according to claim 1, wherein the thin film is formed by evaporation of stearic acid or a paraffin wax.

4. The microchip according to claim 3, wherein the at least one of the substances present in the reaction region is at least one selected from an oligonucleotide primer, an enzyme, and a nucleic acid monomer.

5. The microchip according to claim 2, wherein an oligonucleotide primer is fixed on the thin film covering an enzyme.

6. The microchip according to claim 1, wherein the microchip includes

an inlet into which a liquid is introduced from the outside,

a plurality of the reaction regions, and

a channel configured to supply the liquid introduced from the inlet to the reaction regions.

7. A method for producing a microchip for an isothermal nucleic-acid amplification reaction, comprising:

covering at least one of substances necessary for an isothermal amplification reaction of a nucleic acid, the at least one of the substances being placed in a reaction region functioning as a reaction field of the reaction, with a thin film that melts at a temperature higher than room temperature and lower than a reaction temperature of the reaction.

8. An isothermal nucleic-acid amplification method comprising:

into a reaction region where at least one of substances necessary for an isothermal amplification reaction of a nucleic acid is present, the at least one of the substances being covered with a thin film that melts at a temperature higher than room temperature and lower than a reaction temperature of the reaction, introducing the remaining substances necessary for the reaction; and

increasing the temperature to the reaction temperature.