CELL CULTURE METHOD, CELL CULTURE DEVICE, METHOD FOR COUNTING SUBSJECT MATTERS TO BE COUNTED IN CONTAINER AND DEVICE FOR COUNTING
First Claim
1. A method for culturing cells using a culture container, the method comprising applying an external force to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells disposed in a culture solution in the culture container.
2 Assignments
0 Petitions
Accused Products
Abstract
Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells.
An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container. A method for counting counted targets disposed in a liquid enclosed in a container includes adjusting a thickness of at least a part of the container. At least a part of the adjusted part is set as a measurement target region. A number of counted targets in the measurement target region is counted.
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Citations
20 Claims
- 1. A method for culturing cells using a culture container, the method comprising applying an external force to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells disposed in a culture solution in the culture container.
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5. A cell culturing apparatus comprising:
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a loading base on which a cell container is placed, the cell container enclosing a culture solution comprising a culture medium and cells; and an agitating member configured to press the culture container to a predetermined pressing degree and movable at a predetermined speed in a horizontal direction, wherein the agitating member is configured to move to agitate the culture solution in the culture container so as to control at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container. - View Dependent Claims (6, 7, 8)
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9. A method for counting counted targets disposed in a liquid enclosed in a container, the method comprising:
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adjusting a thickness of at least a part of the container;
setting at least a part of the adjusted part as a measurement target region; andcounting a number of counted targets in the measurement target region. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17)
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18. A counting apparatus to count counted targets disposed in a liquid enclosed in a container, the counting apparatus comprising:
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a loading base on which the container is placed; and an adjusting member configured to adjust at least a part of the container including a measurement target region to a predetermined thickness. - View Dependent Claims (19, 20)
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Specification