Signal Multiplexing and Signal Amplification
First Claim
Patent Images
1. A method of amplifying a nucleic acid detection signal, which comprises:
- hybridizing one or more label extender probes to a target nucleic acid;
hybridizing a pre-amplifier to the one or more label extender probes;
hybridizing one or more amplifiers to the pre-amplifier;
hybridizing one or more label spoke probes to the one or more amplifiers; and
hybridizing one or more label probes to the one or more label spoke probes.
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Abstract
Disclosed are methods, compositions and kits for amplifying signals for detecting the presence, quantity and/or sequence of nucleic acids and proteins, as well as methods, compositions and kits for increasing the number of such targets simultaneously detectable in a sample. Detection may be, for instance, in vivo, in cellulo or in situ. Amplification of signal is achieved by way of hybridization of nucleic acid label probe systems and structures. Increase in target multiplex capacity is achieved by way of varying the type of labels utilized in the nucleic acid label probe system.
22 Citations
15 Claims
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1. A method of amplifying a nucleic acid detection signal, which comprises:
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hybridizing one or more label extender probes to a target nucleic acid; hybridizing a pre-amplifier to the one or more label extender probes; hybridizing one or more amplifiers to the pre-amplifier; hybridizing one or more label spoke probes to the one or more amplifiers; and hybridizing one or more label probes to the one or more label spoke probes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of amplifying a protein detection signal, which comprises:
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hybridizing a molecule possessing affinity for the target protein, wherein the molecule comprises covalently conjugated thereto a pre-amplifier probe; hybridizing one or more amplifiers to the pre-amplifier; hybridizing one or more label spoke probes to the one or more amplifiers; and hybridizing one or more label probes to the one or more label spoke probes. - View Dependent Claims (11, 12, 13, 14, 15)
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Specification