Enhanced Clarification Media

• US 20120006751A1
• Filed: 05/06/2011
• Published: 01/12/2012
• Est. Priority Date: 05/07/2010
• Status: Abandoned Application

• Current Assignee: EMD Millipore Corporation (Merck & Co., Inc.)
• Original Assignee: Millipore Corporation (Merck & Co., Inc.)
• Inventors: Gagnon, Brian, Kozlov, Mikhail, Ramaswamy, Senthil, Woo, Maybelle, Cheng, KS
• Application Number:
  US13/102,079
• Publication Number:
  US 20120006751A1
• Time in Patent Office: Days
• US Class Current: 210/660
• CPC Class Codes: B01D 15/00 Separating processes involving the treatment of liquids with solid sorbents using liquid sorbents B01D11/00; ion exchange processes or materials, sorbent materials in general B01J, e.g. sorbents for chromatography B01J20/281; for investigating or analysing materials G01N30/00; Apparatus therefor
• CPC Class Codes: B01D 2239/0407 comprising particulate additives, e.g. adsorbents
• CPC Class Codes: B01D 2239/0464 Impregnants
• CPC Class Codes: B01D 39/18 the material being cellulose or derivatives thereof cork or peat B01D39/1646 ; making filter paper D21F11/14
• CPC Class Codes: B01J 20/14 Diatomaceous earth
• CPC Class Codes: B01J 20/28023 Fibres or filaments fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence
• CPC Class Codes: B01J 20/2803 Sorbents comprising a binder, e.g. for forming aggregated, agglomerated or granulated products
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3282 Crosslinked polymers
• CPC Class Codes: B01J 2220/46 Materials comprising a mixture of inorganic and organic materials materials coated or impregnated on a carrier B01J20/32

Abstract

Media and devices, such as depth filters including such media, wherein the media is impregnated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

Claims

1. A porous sorptive media comprising cellulose impregnated with a crosslinked polymer having attached primary amine groups.

2. The porous sorptive media of claim 1, further comprising diatomaceous earth.

3. The porous coated media of claim 1, wherein said crosslinked polymer comprises polyallylamine or a protonated polyallylamine.

4. The porous sorptive media of claim 1, wherein said crosslinked polymer comprises a copolymer or block copolymer containing polyallylamine or a protonated polyallylamine.

5. The porous sorptive media of claim 1, wherein said cellulose comprises cellulose fibers.

6. The porous sorptive media of claim 2, wherein said cellulose and said diatomaceous earth are held together by a binder.

7. A depth filter comprising a housing containing a matrix of cellulose fibers impregnated with a crosslinked polymer having attached primary amine groups.

8. The depth filter of claim 7, wherein said matrix further comprises diatomaceous earth.

9. The depth filter of claim 7, wherein said crosslinked polymer comprises polyallylamine or a protonated polyallylamine.

10. The depth filter of claim 7, wherein said crosslinked polymer comprises a copolymer or block copolymer containing polyallylamine or a protonated polyallylamine.

11. The depth filter of claim 8, wherein said cellulose fibers and said diatomaceous earth are held together by a binder.

12. A method of removing impurities from a biological sample, comprising filtering said sample through porous sorptive media comprising cellulose impregnated with a crosslinked polymer having attached primary amine groups.

13. The method of claim 12, wherein said biological sample comprises a solution having a pH of about 7.5.

14. The method of claim 12, wherein said biological sample comprises a solution having a conductivity of about 10.4 mS/cm.