Genome editing of a Rosa locus using zinc-finger nucleases
First Claim
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1. A fusion protein comprising a nuclease and an engineered zinc finger domain, wherein the engineered zinc finger domain binds to a target site specified in any of SEQ ID NOs:
- 28-30 and 75-90.
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Abstract
Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
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29 Claims
- 1. A fusion protein comprising a nuclease and an engineered zinc finger domain, wherein the engineered zinc finger domain binds to a target site specified in any of SEQ ID NOs:
Specification