Sample Processing Droplet Actuator, System and Method
First Claim
1. A method of dispensing a sample droplet, the method comprising:
- (a) providing a droplet actuator comprising substrates arranged to form a gap, wherein;
(i) the gap comprises;
(1) a parent droplet comprising a protein in a concentration exceeding about 10 mg/mL;
(2) an oil filler fluid surrounding the droplet, the filler fluid comprising a surfactant in an amount selected to enhance droplet operations using the sample; and
(ii) one or more of the substrates comprise electrodes arranged for conducting droplet operations in the gap by electrowetting using the droplet; and
(b) using the electrodes to conduct the droplet operations comprising dispensing sub-droplets from the parent droplet.
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Accused Products
Abstract
Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
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Citations
23 Claims
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1. A method of dispensing a sample droplet, the method comprising:
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(a) providing a droplet actuator comprising substrates arranged to form a gap, wherein; (i) the gap comprises; (1) a parent droplet comprising a protein in a concentration exceeding about 10 mg/mL; (2) an oil filler fluid surrounding the droplet, the filler fluid comprising a surfactant in an amount selected to enhance droplet operations using the sample; and (ii) one or more of the substrates comprise electrodes arranged for conducting droplet operations in the gap by electrowetting using the droplet; and (b) using the electrodes to conduct the droplet operations comprising dispensing sub-droplets from the parent droplet. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification