Fluorescent Methods and Materials for Directed Biomarker Signal Amplification
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Abstract
Methods and compositions are provided that include a multichromophore and/or multichromophore complex for identifying a target biomolecule. A sensor biomolecule, for example, an antibody can be covalently linked to the multichromophore. Additionally, a signaling chromophore can be covalently linked to the multichromophore. The arrangement is such that the signaling chromophore is capable of receiving energy from the multichromophore upon excitation of the multichromophore. Since the sensor biomolecule is capable of interacting with the target biomolecule, the multichromophore and/or multichromophore complex can provide enhanced detection signals for a target biomolecule.
49 Citations
96 Claims
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1-71. -71. (canceled)
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72. A multichromophore complex comprising:
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a multichromophore coupled to at least one biomolecule selected from the group consisting of a sensor biomolecule, a bioconjugate and a target biomolecule wherein, the multichromophore is covalently bound by a bioconjugation site pendant, a signaling chromophore that is the multichromophore or is covalently bound to the multichromophore complex, and the multichromophore is a substituted conjugated polymer, a conjugated polymer segment or an oligomeric structure.
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86. A multichromophore complex for identifying a target biomolecule comprising:
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a multichromophore comprising an optionally substituted conjugated polymer, conjugated polymer segment or oligomeric structure, a sensor biomolecule covalently linked to the multichromophore, a signaling chromophore that is the multichromophore, covalently bound to the multichromophore, or covalently bound to the sensor biomolecule, wherein the signaling chromophore, except when the signaling chromophore is the multichromophore, is capable of receiving energy from the multichromophore upon excitation of the multichromophore and the sensor biomolecule is capable of interacting with the target biomolecule or a target-associated biomolecule.
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96. An assay method comprising:
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providing a sample that is suspected of containing a target biomolecule; providing a multichromophore complex comprising a multichromophore, covalently bound to a biomolecule, and a signaling chromophore covalently linked to the multichromophore complex, wherein the multichromophore comprises an optionally substituted conjugated polymer, conjugated polymer segment, or oligomeric structure, the signaling chromophore is capable of receiving energy from the multichromophore upon excitation of the multichromophore and the sensor biomolecule is capable of interacting with the target biomolecule or a target-associated biomolecule; contacting the sample with the multichromophore complex under conditions in which the sensor biomolecule can bind to the target biomolecule or a target-associated biomolecule if present; applying a light source to the sample that can excite the multichromophore; and detecting whether light is emitted from the signaling chromophore.
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Specification