CHIMERIC NK RECEPTOR AND METHODS FOR TREATING CANCER

• US 20120029063A1
• Filed: 06/08/2011
• Published: 02/02/2012
• Est. Priority Date: 09/24/2004
• Status: Active Grant

• Current Assignee: The Trustees of Dartmounth College (Dartmouth College)
• Original Assignee: The Trustees of Dartmounth College (Dartmouth College)
• Inventors: ZHANG, TONG, SENTMAN, CHARLES L.
• Granted Patent: US 8,252,914 B2
• Time in Patent Office: Days
• US Class Current: 514/44.R
• CPC Class Codes: A61K 2239/31 characterized by the route of administration
• CPC Class Codes: A61K 2239/38 characterised by the dose, timing or administration schedule
• CPC Class Codes: A61K 2239/48 Blood cells, e.g. leukemia or lymphoma
• CPC Class Codes: A61K 35/17 Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes when activated by a specific antigen A61K39/00
• CPC Class Codes: A61K 38/10 Peptides having 12 to 20 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 39/4611 T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
• CPC Class Codes: A61K 39/464429 Molecules with a "CD" designation not provided for elsewhere
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 14/715 for cytokines; for lymphokines; for interferons

Abstract

The present invention relates to chimeric immune receptor molecules for reducing or eliminating tumors. The chimeric receptors are composed a C-type lectin-like natural killer cell receptor, or a protein associated therewith, fused to an immune signaling receptor containing an immunoreceptor tyrosine-based activation motif. Methods for using the chimeric receptors are further provided.

Claims

1. A nucleic acid construct for expressing a chimeric polypeptide comprising:

a first nucleic acid sequence encoding a promoter operably linked to

a second nucleic acid sequence encoding a chimeric polypeptide comprising DAP10 fused to a nucleic acid encoding an immune signaling receptor containing an immunoreceptor tyrosine-based activation motif of SEQ ID NO;

1.

2. The nucleic acid construct of claim 1, wherein the construct is in a vector.

3. The nucleic acid construct of claim 1 further comprising a suicide gene.

5. An isolated T cell comprising the nucleic acid construct of claim 1 or 3, or the vector of claim 2.

4. (canceled)