Method of Continuously Measuring Substrate Concentration

• US 20120029332A1
• Filed: 03/15/2010
• Published: 02/02/2012
• Est. Priority Date: 03/16/2009
• Status: Abandoned Application

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Tsukada, Masashi
• Application Number:
  US13/256,525
• Publication Number:
  US 20120029332A1
• Time in Patent Office: Days
• US Class Current: 600/345
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: A61B 5/4839 combined with drug delivery
• CPC Class Codes: A61B 5/6849 in combination with a needle set
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

Analysis equipment is provided, which is capable of fulfilling a demand for miniaturization and ensuring high sensitivity, and which can be produced easily. The present invention relates to a method of continuously measuring a substrate concentration based on a response when a voltage is applied to a sensor. The present invention includes a response voltage application step of applying a response voltage E2 at which a response attributed to a substrate is obtained and a non-response voltage application step of applying a non-response voltage E1 at which the response attributed to the substrate is not obtained or is not substantially obtained. Preferably, the response voltage application step and the non-response voltage application step are repeated alternately.

Claims

1. A method of continuously measuring a substrate concentration based on a response when a voltage is applied to a sensor, comprising:

a response voltage application step of applying a response voltage at which a response attributed to a substrate is obtained; and

a non-response voltage application step of applying a non-response voltage at which the response attributed to the substrate is not obtained or is not substantially obtained.

2. The method of continuously measuring a substrate concentration according to claim 1, wherein the response voltage application step and the non-response voltage application step are repeated alternately.

3. The method of continuously measuring a substrate concentration according to claim 1, wherein the substrate concentration is calculated based on a difference between the response obtained in the response voltage application step and the response obtained in the non-response voltage application step.

4. The method of continuously measuring a substrate concentration according to claim 3, wherein, when the voltage to be applied to the sensor is increased, the non-response voltage is set in a range of −

0.5 to +0.5 V with respect to an applied voltage at which the response attributed to the substrate starts being obtained.

5. The method of continuously measuring a substrate concentration according to claim 2, further comprising a standby step of applying a voltage smaller in an absolute value than the non-response voltage after performing the response voltage application step and the non-response voltage application step alternately a predetermined number of times.

6. The method of continuously measuring a substrate concentration according to claim 2, further comprising a standby step of setting a circuit including the sensor to become an open circuit after performing the response voltage application step and the non-response voltage application step alternately a predetermined number of times.

7. The method of continuously measuring a substrate concentration according to any one of claims 1, wherein the sensor is implanted in a body for use.

8. The method of continuously measuring a substrate concentration according to any one of claims 1, wherein the sensor includes an enzyme-immobilized electrode.

9. The method of continuously measuring a substrate concentration according to any one of claims 1, wherein:

the substrate comprises glucose; and

the sensor comprises a glucose sensor.

10. The method of continuously measuring a substrate concentration according to claim 9, wherein the glucose sensor includes an electrode with a glucose oxidoreductase immobilized thereon.

11. The method of continuously measuring a substrate concentration according to claim 2, wherein the substrate concentration is calculated based on a difference between the response obtained in the response voltage application step and the response obtained in the non-response voltage application step.