Methods for Predicting Stability and Melting Temperatures of Nucleic Acid Duplexes
First Claim
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1. A method of predicting the melting temperature of an oligonucleotide comprising:
- (a) a computer system receiving a data input from a user, the computer comprising a processor, and instructions executable by the processor;
(b) responsive to the data input from a user, the computer system calculating a Tm value using the equation;
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Abstract
The present invention provides methods that more accurately predict melting temperatures for duplex oligomers. The invented methods predict the Tm of chimeric duplexes containing various amounts of locked nucleic acid modifications in oligonucleotide strands.
95 Citations
22 Claims
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1. A method of predicting the melting temperature of an oligonucleotide comprising:
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(a) a computer system receiving a data input from a user, the computer comprising a processor, and instructions executable by the processor; (b) responsive to the data input from a user, the computer system calculating a Tm value using the equation; - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of predicting the melting temperature of an oligonucleotide comprising:
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(a) a networked server receiving data input from a communication device associated with a user, the server comprising the communication interface, a processor, and instructions executable by the processor; (b) responsive to receiving the data input, the networked server sending via the communication interface to the communication device, calculation of a Tm value using the equation; - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
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15. A method of predicting the stability of an oligonucleotide comprising:
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(a) a computer system receiving a data input from a user, the computer comprising a processor, and instructions executable by the processor; (b) responsive to the data input from a user, the computer system calculating a free energy value using the equation;
Δ
G°
=Δ
G°
DNA+Δ
Δ
G°
LNA; and(c) providing an output to a display, wherein the oligonucleotide comprises at least two Locked Nucleic Acid (LNA) modifications. - View Dependent Claims (16, 17)
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18. A method of predicting the stability of an oligonucleotide comprising:
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(a) a networked server receiving data input from a communication device associated with a user, the server comprising the communication interface, a processor, and instructions executable by the processor; (b) responsive to receiving the data input, the networked server sending via the communication interface to the communication device, calculation of a free energy value using the equation;
Δ
G°
=Δ
G°
DNA+Δ
Δ
G°
LNA; and(c) sending the free energy value via the communication interface to the user communication device, wherein the oligonucleotide comprises at least two Locked Nucleic Acid (LNA) modifications. - View Dependent Claims (19, 20, 21, 22)
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Specification