Transient Decay Amperometry
First Claim
1. A method for determining an analyte concentration in a sample, comprising:
- reacting an analyte in a sample with an ionizing agent;
forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period of at most 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample;
applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, whereat least two of the excitations have a duration from 0.1 to 5 seconds, and whereat least one of the relaxations has a duration from 0.4 to 1 second;
generating an output signal from at least one of the excitations, the output signal having a transient decay responsive to a redox reaction of the measurable species; and
determining the analyte concentration of the sample from the transient decay of the generated output signal.
2 Assignments
0 Petitions
Accused Products
Abstract
A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.
2 Citations
56 Claims
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1. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period of at most 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample; applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, where at least two of the excitations have a duration from 0.1 to 5 seconds, and where at least one of the relaxations has a duration from 0.4 to 1 second; generating an output signal from at least one of the excitations, the output signal having a transient decay responsive to a redox reaction of the measurable species; and determining the analyte concentration of the sample from the transient decay of the generated output signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A biosensor for determining an analyte concentration in a sample, comprising:
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a measurement device having a processor connected to a sensor interface; a sensor strip having a sample interface on a base, the sensor interface in electrical communication with the sample interface, where the sample interface is adjacent to a reservoir formed by the base; where the processor instructs a charger to apply an input signal to the reservoir after an incubation period of at most 8 seconds, where the input signal includes excitations and relaxations, at least two of the excitations have a duration from 0.1 to 5 seconds, and where at least one of the relaxations has a duration from 0.4 to 1 second; and where the processor determines the analyte concentration in the sample from an output signal having a transient decay in response to a redox reaction of the analyte in the sample.
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34-35. -35. (canceled)
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36. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period of at most 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample; applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, where at least one of the excitations has a duration from 0.1 to 1 second, and where at least one of the relaxations has a duration from 0.1 to 3 seconds; generating an output signal from the at least one of the excitations having the duration from 0.1 to 1 second, the output signal having a transient decay responsive to a redox reaction of the measurable species; and determining the analyte concentration of the sample from the transient decay of the generated output signal.
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37-38. -38. (canceled)
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39. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from a redox reaction between a portion of the analyte in the sample and the ionizing agent during an incubation period of at most 8 seconds; applying an input signal to the sample after the incubation period; generating an output signal responsive to the analyte concentration in the sample from the input signal, the output signal having a transient decay responsive to the measurable species; and determining the analyte concentration of the sample from the transient decay of the output signal.
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40-47. -47. (canceled)
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48. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a variant concentration distribution of a measurable species in the sample from a redox reaction between the analyte in the sample and the ionizing agent in a reservoir during an incubation period of at most 8 seconds, where the reservoir is defined by a sensor strip base and a bottom surface of a lid, and where the measurable species concentration in the sample is responsive to an analyte concentration of the sample; applying an input signal through substantially planar working and counter electrodes to the sample after the incubation period, the substantially planar working and counter electrodes within the reservoir; generating an output signal responsive to the analyte concentration in the sample from the input signal, the output signal having a transient decay responsive to the measurable species; and determining the analyte concentration of the sample from the transient decay of the output signal.
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49-54. -54. (canceled)
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55. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from a redox reaction between the analyte in the sample and the ionizing agent during an incubation period of at most 4.75 seconds; applying an input signal to the sample after the incubation period, the input signal including excitations and relaxations, where at least one of the excitations has a duration from 0.1 to 5 seconds and at least one of the relaxations has a duration from 0.1 to 3 seconds; generating an output signal responsive to the analyte concentration in the sample from at least one of the excitations within 0.5 to 5 seconds of reacting the analyte in the sample with the ionizing agent, the output signal having a transient decay of continually decreasing currents responsive to the measurable species; and determining the analyte concentration of the sample from the continually decreasing current decay of the output signal.
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56-57. -57. (canceled)
Specification