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Recombinant Polymerases For Improved Single Molecule Sequencing

  • US 20120034602A1
  • Filed: 07/05/2011
  • Published: 02/09/2012
  • Est. Priority Date: 03/31/2008
  • Status: Active Grant
First Claim
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1. A composition comprising a recombinant Φ

  • 29-type DNA polymerase, which recombinant polymerase comprises a mutation at position E375, a mutation at position K512, and a mutation at one or more positions selected from the group consisting of L253, A484, V250, E239, Y224, Y148, E508, and T368, wherein identification of positions is relative to wild-type Φ

    29 polymerase (SEQ ID NO;

    1).

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