DEVICES, SYSTEMS AND METHODS FOR REDUCING THE CONCENTRATION OF A CHEMICAL ENTITY IN FLUIDS

• US 20120040429A1
• Filed: 10/24/2011
• Published: 02/16/2012
• Est. Priority Date: 06/08/2006
• Status: Active Grant

• Current Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Original Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Inventors: RUSSELL, ALAN J., FEDERSPIEL, WILLIAM J., OH, HEUNG-IL, KAAR, JOEL L.
• Granted Patent: US 8,323,379 B2
• Time in Patent Office: Days
• US Class Current: 435/180
• CPC Class Codes: A61M 1/1698 Blood oxygenators with or without heat-exchangers intracorporal A61M1/1678; manufacturing of membranes therefor B01D67/00; semi-permeable membranes for separation processes characterised by their properties B01D69/00; semi-permeable membranes characterised by their material B01D71/00
• CPC Class Codes: A61M 1/28 Peritoneal dialysis ; Other peritoneal treatment, e.g. oxygenation
• CPC Class Codes: A61M 1/3623 Means for actively controlling temperature of blood
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: B01D 19/0031 by filtration
• CPC Class Codes: B01D 2257/504 Carbon dioxide
• CPC Class Codes: B01D 2311/2653 Degassing
• CPC Class Codes: B01D 2323/30 Cross-linking
• CPC Class Codes: B01D 2325/10 Catalysts being present on the surface of the membrane or in the pores
• CPC Class Codes: B01D 53/1425 Regeneration of liquid absorbents
• CPC Class Codes: B01D 53/228 characterised by specific membranes
• CPC Class Codes: B01D 53/229 Integrated processes (Diffusion and at least one other process, e.g. adsorption, absorption)
• CPC Class Codes: B01D 67/009 with wave-energy, particle-radiation or plasma
• CPC Class Codes: B01D 67/0093 Chemical modification
• CPC Class Codes: B01D 67/00931 by introduction of specific groups after membrane formation, e.g. by grafting
• CPC Class Codes: B01D 69/08 Hollow fibre membranes manufacture of hollow fibres D01D5/24, D01F1/08
• CPC Class Codes: B01D 69/144 containing embedded or bound biomolecules
• CPC Class Codes: B01D 69/145 containing embedded catalysts
• CPC Class Codes: B01D 71/26 Polyalkenes
• CPC Class Codes: B01D 71/261 Polyethylene
• CPC Class Codes: B01D 71/262 : Polypropylene
• CPC Class Codes: B01D 71/70 : Polymers having silicon in the main chain, with or without sulfur, nitrogen, oxygen or carbon only
• CPC Class Codes: Y02C 20/40 : of CO2

Abstract

A method of improving the blood compatibility of a blood-contacting surface includes immobilizing carbonic anhydrase on the surface, wherein the surface exhibits carbonic anhydrase activity of at least 20% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

Claims

1. A method of improving the blood compatibility of a blood-contacting surface comprising immobilizing carbonic anhydrase on the surface, wherein the surface exhibits carbonic anhydrase activity of at least 20% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

2. The method of claim 1 wherein immobilization of carbonic anhydrase on the surface reduces at least one of platelet activation in blood associated with the surface or platelet deposition on the surface.

3. The method of claim 2 wherein the surface is formed from a polymeric material.

4. The method of claim 3 wherein the carbonic anhydrase is immobilized on the polymeric material of the surface via adsorption, covalent bonding, ionic bonding or chelation.

5. The method of claim 3 wherein the carbonic anhydrase is covalently attached to the polymeric material of the surface.

6. The method of claim 5 wherein the polymeric material is microporous or permeable.

7. The method of claim 6 wherein the polymeric material is microporous and sufficiently hydrophobic so that its pores remain gas filled after contacting blood or other aqueous fluids.

8. The method of claim 7 wherein the polymeric material is an olefinic polymeric material.

9. The method of claim 2 wherein the surface exhibits carbonic anhydrase activity of at least 40% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

10. The method of claim 2 wherein the surface exhibits carbonic anhydrase activity of at least 60% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

11. The method of claim 2 wherein the surface exhibits carbonic anhydrase activity of at least 80% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

12. The method of claim 3 wherein the polymeric material is treated prior to immobilizing the carbonic anyhdrase thereon to create reactive sites on the polymeric material.

13. The method of claim 12 wherein the reactive sites comprise at least one of a hydroxyl group, an amine group or a carboxyl group.