CENTRIFUGAL CAPTURE SYSTEM
First Claim
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1. A centrifugal capture chamber configured to receive a fluid comprising particles of interest and being operably rotatable about an axis of rotation, the chamber having an axis of rotation proximal portion and an axis of rotation distal portion, the capture chamber comprising:
- a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber from the axis of rotation proximal portion to the axis of rotation distal portion; and
wherein the chamber is configured such that a rotation of the capture chamber provides a centrifugal force which induces motion on the particles such that they are biased in straight lines in a radial direction away from the axis of rotation of the chamber and are sedimented into the capture areas of the capture sites.
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Abstract
A particle capture system that can be used in the context of a lab-on-a-chip platform for particle- and cell-based assays is described. The system comprises a capture chamber comprising a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber. By rotating the chamber, the individual particles are biased towards the capture sites where they may be captured.
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Citations
54 Claims
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1. A centrifugal capture chamber configured to receive a fluid comprising particles of interest and being operably rotatable about an axis of rotation, the chamber having an axis of rotation proximal portion and an axis of rotation distal portion, the capture chamber comprising:
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a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber from the axis of rotation proximal portion to the axis of rotation distal portion; and wherein the chamber is configured such that a rotation of the capture chamber provides a centrifugal force which induces motion on the particles such that they are biased in straight lines in a radial direction away from the axis of rotation of the chamber and are sedimented into the capture areas of the capture sites. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A system for sedimentary centrifugal capture of particles, the system comprising a rotatable substrate having at least one chamber, the capture chamber being rotatable about an axis of rotation of the substrate, the chamber having an axis of rotation proximal portion and an axis of rotation distal portion, the capture chamber being configured to receive a fluid comprising particles of interest, the capture chamber comprising:
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a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber from the axis of rotation proximal portion to the axis of rotation distal portion; and wherein the chamber is configured such that a rotation of the capture chamber provides a centrifugal force which induces motion on the particles such that they are biased in straight lines in a radial direction away from the axis of rotation and are sedimented into the capture areas of the capture sites. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51)
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52. A method of selectively capturing particles within a microfabricated structure, the method comprising:
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a) Introducing particles within a fluid suspension into a chamber, the chamber comprising a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber, and b) Rotating the chamber about an axis of rotation, the rotation of the chamber providing a centrifugal force which induces motion on the particles such that they are biased in straight lines in a radial direction away from the axis of rotation of the chamber and are sedimented into the capture areas of the capture sites. - View Dependent Claims (53, 54)
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Specification