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Detection of Nucleic Acids

  • US 20120052498A1
  • Filed: 07/01/2011
  • Published: 03/01/2012
  • Est. Priority Date: 07/01/2010
  • Status: Abandoned Application
First Claim
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1. A method of genotyping a single nucleotide polymorphism (SNP), which comprises:

  • providing a sample comprising or suspected of comprising a target nucleic acid comprising at least one SNP;

    incubating at least two label extender probes each comprising a different L-1 sequence, at least two capture extender probes each comprising a different C-1 sequence, and a label probe system with the sample comprising or suspected of comprising the target nucleic acid comprising the at least one SNP, wherein at least one L-1 sequence is perfectly complementary to the at least one allele of a SNP;

    adding one or more subsets of particles comprising covalently bound thereto at least two capture probes, under conditions in which the particles bind to the capture extender probes, wherein the capture probes are covalently bound to the particles, wherein one or more particles within a subset of particles is distinguishable from one or more particles in any other subset of particles;

    detecting whether the particles have bound thereto the label probe system, thereby genotyping the at least one allele of the SNP.

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