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SYSTEM AND METHOD FOR SERIAL PROCESSING OF MULTIPLE NUCLEIC ACID ASSAYS

  • US 20120052560A1
  • Filed: 08/31/2011
  • Published: 03/01/2012
  • Est. Priority Date: 08/31/2010
  • Status: Active Grant
First Claim
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1. A system for serial processing of nucleic acid assays, comprising:

  • a microfluidic cartridge comprising an interface chip having at least one inlet port and microfluidic channel and a reaction chip having at least one microfluidic channel in fluid communication with an associated microfluidic channel of the interface chip;

    a flow control module configured to control assay fluid flow through the at least one microfluidic channel and to selectively apply pneumatic pressures to the at least one microfluidic channel of the interface chip and the reaction chip;

    said flow control module being configured to;

    (a) draw a first fluid into the microfluidic channel of the interface chip via the inlet port of the interface chip;

    (b) create a first fluid segment in the microfluidic channel of the reaction chip by drawing the first fluid from the associated microfluidic channel of the interface chip into the microfluidic channel of the reaction chip;

    (c) draw a second fluid into the microfluidic channel of the interface chip via the inlet port of the interface chip while maintaining the position of the first fluid segment in the reaction chip; and

    (d) create a second fluid segment in the microfluidic channel of the reaction chip by drawing the second fluid from the associated microfluidic channel of the interface chip into the microfluidic channel of the reaction chip;

    a temperature measurement and control system configured to control and measure a temperature of one or more portions of the at least microfluidic channel of the reaction chip; and

    a fluorescence imaging system comprising one or more excitation elements and an imaging sensor and configured to create images of fluorescent emissions from materials within the at least one microfluidic channel of the reaction chip.

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