TEMPERING OF CELLULOSIC BIOMASS
First Claim
1. A method for cost-effective processing of lignocellulosic biomass comprising steps of:
- tempering a sample of plant biomass under conditions to promote activation of lignocellulolytic enzyme polypeptides present in the sample of plant biomass;
pretreating the sample under conditions to promote accessibility of celluloses within the lignocellulosic biomass; and
treating the pretreated sample under conditions that promote hydrolysis of cellulose to fermentable sugars,wherein the sample of plant biomass is obtained from at least one transgenic plant, the genome of which is augmented with;
a recombinant polynucleotide encoding at least one lignocellulolytic enzyme polypeptide operably linked to a promoter sequence, wherein the polynucleotide is optimized for expression in the plant.
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Abstract
The present invention is directed to improved systems and methods for reducing costs and increasing yields of cellulosic ethanol. In particular, the present invention provides plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Also provided are methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols that are typically employed. Such methods allow, among other things, reduced costs associated with externally applied lignocellulolytic enzyme polypeptides.
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Citations
49 Claims
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1. A method for cost-effective processing of lignocellulosic biomass comprising steps of:
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tempering a sample of plant biomass under conditions to promote activation of lignocellulolytic enzyme polypeptides present in the sample of plant biomass; pretreating the sample under conditions to promote accessibility of celluloses within the lignocellulosic biomass; and treating the pretreated sample under conditions that promote hydrolysis of cellulose to fermentable sugars, wherein the sample of plant biomass is obtained from at least one transgenic plant, the genome of which is augmented with; a recombinant polynucleotide encoding at least one lignocellulolytic enzyme polypeptide operably linked to a promoter sequence, wherein the polynucleotide is optimized for expression in the plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification