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TEMPERING OF CELLULOSIC BIOMASS

  • US 20120058523A1
  • Filed: 02/17/2010
  • Published: 03/08/2012
  • Est. Priority Date: 02/17/2009
  • Status: Abandoned Application
First Claim
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1. A method for cost-effective processing of lignocellulosic biomass comprising steps of:

  • tempering a sample of plant biomass under conditions to promote activation of lignocellulolytic enzyme polypeptides present in the sample of plant biomass;

    pretreating the sample under conditions to promote accessibility of celluloses within the lignocellulosic biomass; and

    treating the pretreated sample under conditions that promote hydrolysis of cellulose to fermentable sugars,wherein the sample of plant biomass is obtained from at least one transgenic plant, the genome of which is augmented with;

    a recombinant polynucleotide encoding at least one lignocellulolytic enzyme polypeptide operably linked to a promoter sequence, wherein the polynucleotide is optimized for expression in the plant.

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