Microarray system

• US 20120071355A9
• Filed: 05/09/2008
• Published: 03/22/2012
• Est. Priority Date: 05/09/2008
• Status: Active Grant

• Current Assignee: Akonni Biosystems, Inc.
• Original Assignee: Akonni Biosystems, Inc.
• Inventors: Cooney, Christopher G.
• Granted Patent: US 8,680,025 B2
• Time in Patent Office: Days
• US Class Current: 506/16
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides

Abstract

A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.

Claims

1. A microarray system, comprising:

a microarray formed on a planar substrate; and

an incubation chamber formed around said microarray,

wherein said incubation chamber comprises a plurality of interior surfaces including a bottom surface on which said microarray is formed and a top surface that faces said microarray, and

wherein at least one of said a plurality of interior surfaces is a hydrophilic surface.

2. The microarray system of claim 1, wherein said hydrophilic surface is said top surface.

3. The microarray system of claim 2, wherein said hydrophilic surface is formed by covering said top surface with a hydrophilic coating.

4. The microarray system of claim 2, wherein said incubation chamber is formed by placing a gasket around said microarray and covering said gasket with a hydrophilic tape or a hydrophilic film.

5. The microarray system of claim 4, wherein said hydrophilic tape or hydrophilic film is transparent.

6. The microarray system of claim 1, further comprising a cover slip that covers said planar substrate, wherein said microarray is formed in a recession area on said planar substrate and wherein said incubation chamber is formed between said cover slip and said recession area on said planar substrate.

7. The microarray system of claim 1, further comprising a cover slip that covers said planar substrate, wherein said cover slip has a recession area, said recession area is larger than said microarray and is positioned on top of said microarray, and wherein said incubation chamber is formed between said microarray and said recession area on said cover slip.

8. The microarray system of claim 1, wherein said hydrophilic surface comprises impregnated chemicals that lyses cell membranes.

9. The microarray system of claim 8, wherein said hydrophilic surface comprises a hydrophilic matrix that retains nucleic acid from lysed cells.

10. The microarray system of claim 8, wherein said hydrophilic surface is said top surface.

11. The microarray system of claim 8, wherein said hydrophilic surface is said bottom surface.

12. The microarray system of claim 1, wherein said hydrophilic surface is said bottom surface.

13. The microarray system of claim 1, further comprising a one-way valve for loading a liquid sample into said incubation chamber.

14. The microarray system of claim 13, wherein said one-way valve is a check valve.

15. The microarray system of claim 13, wherein said one-way valve is a dome valve.

16. The microarray system of claim 13, wherein said one-way valve is connected to said incubation chamber through a first channel.

17. The microarray system of claim 1, further comprising a waste chamber.

18. The microarray system of claim 17, wherein said waste chamber comprises an absorbent capable of wicking liquid from said incubation chamber.

19. The microarray system of claim 18, wherein said absorbent comprises cellulose.

20. The microarray system of claim 17, wherein said waste chamber has a volume that is larger than a volume of said incubation chamber.

21. The microarray system of claim 17, wherein said waste chamber is connected to said incubation chamber through a second channel.

22. The microarray system of claim 21, wherein said waste chamber comprises an absorbent placed at a distance from said second channel to control wicking rate.

23. The microarray system of claim 21, wherein said second channel comprises an inlet section, a funnel shape connecting section, and an outlet section, wherein said inlet section has a diameter that is larger than a diameter of said outlet section.

24. The microarray system of claim 17, wherein said waste chamber is vented to atmosphere through a venting channel.

25. The microarray system of claim 1, wherein said substrate is glass.

26. The microarray system of claim 1, wherein said substrate is plastic.

27. The microarray system of claim 1, wherein said microarray is an oligonucleotide array.

28. The microarray system of claim 1, wherein said microarray is a protein array.

29. The microarray system of claim 28, wherein said protein array is an antibody array.

30. The microarray system of claim 1, wherein said microarray is formed by a gel spot printing method.

31. A microarray system, comprising:

a microarray formed on a planar substrate;

an incubation chamber, wherein said incubation chamber surrounds said micro array;

a dome valve for loading a liquid sample into said incubation chamber; and

a channel connecting said one-way valve to said incubation chamber.

32. A microarray system, comprising:

a microarray formed on a planar substrate;

an incubation chamber, wherein said incubation chamber surrounds said micro array;

a waste chamber containing an absorbent; and

a channel connecting said waste chamber to said incubation chamber.

33. The microarray system of claim 32, wherein said incubation chamber comprises an hydrophilic interior surface.