APPARATUS FOR MEASURING BIOMATERIAL AND METHOD FOR MANUFACTURING SAME

• US 20120073967A1
• Filed: 04/30/2010
• Published: 03/29/2012
• Est. Priority Date: 06/02/2009
• Status: Active Grant

• Current Assignee: Ceragem Medisys Inc.
• Original Assignee: Ceragem Medisys Inc.
• Inventors: Lee, Jin-Woo, Choi, Jae-Kyu
• Granted Patent: US 8,753,493 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention relates to an apparatus for measuring biomaterial and a method for manufacturing thereof. The apparatus of the present invention comprises: a first substrate having a recess in one side thereof; a second substrate having a plurality of reaction electrodes where a biochemical reaction of a biomaterial occurs, and a plurality of delivery electrodes delivering signals from the reaction to a detector; and reaction reagents located in the recess causing the reaction with the biomaterial. The second substrate is attached to the first substrate such that a portion of the recess forms a sample-inlet, the recess cooperates with at least one edge of the second substrate to form at least one vent slit, and the reaction electrodes are directed toward the recess. Such apparatus of the present invention enables air in the capillary to be thoroughly and quickly discharged to the outside with biomaterial-introduction, thereby increasing the speed of the biomaterial-introduction.

Claims

1. An apparatus for measuring a biomaterial, comprising:

a first substrate having a recess formed in one side surface thereof;

a second substrate having a plurality of reaction electrodes in which a biochemical reaction of a sample occurs and a plurality of delivery electrodes transmitting a signal generated by the biochemical reaction to a detector; and

a reaction reagent located in the recess and causing the biochemical reaction with the sample,

wherein the second substrate is attached to the first substrate such that the reaction electrodes are directed toward the recess and the recess forms at least one vent slit in combination with at least one edge surface of the second substrate, and

the first and second substrate are attached to form a sample inlet and a reaction chamber.

2. The apparatus according to claim 1, wherein the vent slit continuously extends from the sample inlet.

3. The apparatus according to claim 1, wherein the vent slit is formed in a lengthwise direction of the sample inlet.

4. The apparatus according to claim 1, wherein the second substrate has the reaction electrodes and the delivery electrodes formed on one and the other surfaces thereof;

respectively; and

the reaction electrodes and the delivery electrodes are electrically connected to each other.

5. The apparatus according to claim 4, wherein the delivery electrodes are electrically connected to the reaction electrodes via conductors passing through the second substrate.

6. The apparatus according to claim 4, wherein the delivery electrodes are electrically connected to the reaction electrodes via conductive clamping members.

7. The apparatus according to claim 1, wherein the reaction electrodes and the delivery electrodes are formed on the same surface of the second substrate.

8. The apparatus according to claim 1, further comprising a vent hole formed in the first or second substrate on an opposite side of the sample inlet.

9. The apparatus according to claim 1, wherein the reaction reagent is immobilized to the reaction electrodes.

10. An apparatus for measuring a biomaterial, comprising:

a first substrate having a recess formed in one side surface thereof;

a second substrate;

a plurality of electrodes attached to the first or second substrate; and

a reaction reagent located in the recess and causing the biochemical reaction with the sample,

wherein the second substrate is attached to the first substrate such that the recess forms at least one vent slit in combination with at least one edge surface of the second substrate.

11. A method of manufacturing an apparatus for measuring a biomaterial, comprising:

manufacturing a first substrate having a recess formed in one surface thereof;

manufacturing a second substrate having a plurality of reaction electrodes in which a biochemical reaction of a sample occurs and a plurality of delivery electrodes transmitting a signal generated by the biochemical reaction to a detector;

immobilizing a reaction reagent causing the biochemical reaction with the sample to the first or second substrate so as to be located in the recess; and

attaching the second substrate to the first substrate such that the reaction electrodes are directed toward the recess, and the recess forms at least one vent slit in combination with at least one edge surface of the second substrate, thereby forming a sample inlet and a reaction chamber.

12. The method according to claim 11, wherein the first substrate is manufactured by injection molding, extrusion molding, or plastic laminating.