Electrochemical Test Device

• US 20120073986A1
• Filed: 03/30/2010
• Published: 03/29/2012
• Est. Priority Date: 03/31/2009
• Status: Abandoned Application

• Current Assignee: Diamatrix Incorporated
• Original Assignee: Diamatrix Incorporated
• Inventors: Jackson, James Richard, Palmer, Richard F.
• Application Number:
  US13/262,589
• Publication Number:
  US 20120073986A1
• Time in Patent Office: Days
• US Class Current: 205/775
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

An assay module for an electrochemical test device, said assay module comprising: at least one channel for transporting a liquid to be tested from a deposition zone, where a sample of said liquid to be tested can be deposited, to a testing zone, the testing zone being spaced apart from said deposition zone; and electrodes for measuring an electrical property of liquid in said testing zone, an electrochemical test device comprising such an assay module and a method of testing a liquid sample using such an electrochemical test device.

Claims

1. An assay module for an electrochemical test device, said assay module comprising:

at least one channel for transporting a liquid to be tested from a deposition zone, where a sample of said liquid to be tested can be deposited, to a testing zone, the testing zone being spaced apart from said deposition zone; and

electrodes for measuring an electrical property of liquid in said testing zone.

2. An assay module according to claim 1, wherein said at least one channel comprises a porous material capable of transporting said liquid chromatographically.

3. An assay module according to claim 1, wherein said at least one channel is configured to transport said liquid by capillary action.

4. An assay module according to claim 2, further comprising a doping polymer impermeable to the liquid which the porous material is capable of transporting, incorporated into a part of the porous material so as to define a channel in the body of the porous material, the deposition zone and testing zone being provided on the porous material in the channel defined therein.

5. An assay module according to claim 2, wherein at least one of said electrodes is formed by a printing technique onto said porous material.

6. An assay module according to claim 5, wherein said printing technique is screen printing or ink-jet printing.

7. An assay module according to claim 2, further comprising at least one conductive track between at least one of said electrodes and a contact point on said assay module away from said testing zone, so as to provide an electrical connection between said contact point and said testing zone.

8. An assay module according to claim 7 when dependent on claim 4, further comprising a water-impermeable coating polymer impregnated into the porous material so as to at least partially encapsulate said at least one conductive track and/or said channel.

9. An assay module according to claim 8, wherein said impregnating polymer is applied to both sides of said porous material.

10. An assay module according to claim 7 when dependent on claim 4, wherein said conductive track is formed over said doping polymer defining said channel.

11. An assay module according to claim 1, configured such that movement of said liquid to be tested from said deposition zone to said testing zone causes movement of one or more reagents to said testing zone, at least one of said reagents being such as to modify the electrical properties of liquid in said testing zone if a target analyte is present in said liquid to be tested.

12. An assay module according to claim 11, wherein a least one of said reagents is such as to become immobilized at said testing zone if a target analyte is present in said liquid to be tested, said immobilization of said at least one of said reagents being such as to modify an electrical property of liquid in said testing zone.

13. An assay module according to claim 11, wherein said one or more reagents is/are provided in said one or more channels at a position or positions between said testing zone and said deposition zone, prior to deposition of said liquid to be tested.

14. An assay module according to claim 13, wherein at least one of said one or more reagents is/are so provided in dry form.

15. An assay module according to claim 12, wherein said one or more channels is/are configured to control the movement and/or action of said liquid to be tested and of said one or more reagents, and to deliver said liquid to be tested and said one or more reagents to said testing zone in an appropriate order and with appropriate timing to facilitate the performing of an electrochemical test in said testing zone using said electrodes.

16. An assay module according to claim 1, wherein said electrodes and/or conductive tracks leading to said electrodes are formed by printing of a conductive ink and wherein said ink comprises at least one reagent which facilitates or is necessary for the electrochemical reaction due to take place at the testing zone.

17. An assay module according to claim 1, configured to detect one of the following:

pregnancy from a liquid sample comprising human urine or blood;

drugs of abuse from a liquid sample comprising urine, blood, saliva or test solutions used to process said samples prior to testing;

infectious diseases from a liquid sample comprising urine, blood, saliva or test solutions used to process said samples prior to testing.

18. An electrochemical test device, comprising:

an assay module according to claim 1; and

an analysis and display module comprising;

a measurement section for interacting with said electrodes in said assay module in order to measure an electrical property of liquid in said testing zone;

an analysis section for obtaining information about the composition of said liquid to be tested based on said electrical property of liquid in said testing zone measured by said measurement section; and

a display section for displaying said information obtained by said analysis section.

19. An electrochemical test device according to claim 18, wherein said display section is configured to display information obtained by said analysis section in textual and/or numerical form.

20. An electrochemical test device according to claim 18, wherein said analysis section is capable of detecting at least three distinct concentrations or concentration ranges for a component in said liquid to be tested.

21. An electrochemical test device according to claim 18, wherein said display section is configured to display at least three different images corresponding to each of the at least three distinct concentrations or concentration ranges detected by said analysis section.

22. An electrochemical test device according to claim 18, wherein said analysis and display section comprises a reagent management system for introducing reagent to said assay module via respective reagent interfaces on said analysis and display section and said assay module.

23. An electrochemical test device according to claim 18, wherein said measurement section and electrodes are configured to measure the current between the electrodes at a constant potential difference.

24. An electrochemical test device according to claim 18, wherein said assay module and said analysis and display module each comprise an interface, said assay module and said analysis and display module being detachably connected to each other via said interface.

25. An electrochemical test device according to claim 18, further comprising:

a liquid reservoir for containing a liquid; and

a release mechanism for releasing said liquid into at least one of said at least one channel.

26. A method of testing a liquid sample to determine information about the composition of said liquid sample using an electrochemical test device according to claim 18, said method comprising:

depositing said liquid sample in said deposition zone; and

reading information displayed on said display section.

27. A method according to claim 26, wherein said assay module and said analysis and display module each comprise an interface, said assay module and said analysis and display module being detachably connected to each other via said interface, further comprising:

between said depositing and reading steps, connecting said assay module to said analysis and display module, via said interface.