BIOMIMETIC MEMBRANES AND USES THEREOF

• US 20120080377A1
• Filed: 10/04/2011
• Published: 04/05/2012
• Est. Priority Date: 06/19/2009
• Status: Abandoned Application

• Current Assignee: Aquaporin A/S
• Original Assignee: Aquaporin A/S
• Inventors: Jensen, Peter Holme, Hansen, Jesper Sondergaard, Vissing, Thomas, Perry, Mark Edward, Nielsen, Claus Helix
• Application Number:
  US13/252,449
• Publication Number:
  US 20120080377A1
• Time in Patent Office: Days
• US Class Current: 210/643
• CPC Class Codes: A61M 1/16 with membranes
• CPC Class Codes: A61M 1/1654 Dialysates therefor
• CPC Class Codes: A61M 1/1656 Apparatus for preparing dialysates
• CPC Class Codes: A61M 1/1666 by dissolving solids
• CPC Class Codes: A61M 1/1672 using membrane filters, e.g. for sterilising the dialysate
• CPC Class Codes: A61M 1/1676 containing proteins, e.g. albumin
• CPC Class Codes: B01D 11/0415 in combination with membranes
• CPC Class Codes: B01D 11/0446 Juxtaposition of mixers-settlers
• CPC Class Codes: B01D 11/0492 Applications, solvents used
• CPC Class Codes: B01D 2325/39 Amphiphilic membranes
• CPC Class Codes: B01D 61/002 Forward osmosis or direct osmosis
• CPC Class Codes: B01D 61/246 Membrane extraction
• CPC Class Codes: B01D 61/38 Liquid-membrane separation
• CPC Class Codes: B01D 61/40 using emulsion-type membranes
• CPC Class Codes: B01D 61/58 Multistep processes
• CPC Class Codes: B01D 63/02 Hollow fibre modules
• CPC Class Codes: B01D 63/04 comprising multiple hollow fibre assemblies
• CPC Class Codes: B01D 63/10 Spiral-wound membrane modules
• CPC Class Codes: B01D 69/02 characterised by their properties
• CPC Class Codes: B01D 69/06 Flat membranes
• CPC Class Codes: B01D 69/08 : Hollow fibre membranes manufacture of hollow fibres D01D5/24, D01F1/08
• CPC Class Codes: B01D 69/10 : Supported membranes; Membrane supports
• CPC Class Codes: B01D 69/144 : containing embedded or bound biomolecules
• CPC Class Codes: C02F 1/26 : by extraction
• CPC Class Codes: C02F 1/44 : by dialysis, osmosis or reverse osmosis general membrane separation processes B01D61/00, membrane modules B01D63/00, electrodialysis C02F1/4693, combination of membrane modules and bioreactors C02F3/1268
• CPC Class Codes: C02F 1/442 : by nanofiltration
• CPC Class Codes: C02F 1/444 : by ultrafiltration or microfiltration
• CPC Class Codes: C02F 1/445 : by forward osmosis
• CPC Class Codes: C02F 2101/10 : Inorganic compounds
• CPC Class Codes: C02F 2103/08 : Seawater, e.g. for desalination
• CPC Class Codes: G01N 33/582 : with fluorescent label
• CPC Class Codes: Y02A 20/131 : Reverse-osmosis

Abstract

A liquid membrane system is disclosed in the form of a biochannel containing bulk liquid membrane (BLM), biochannel containing emulsion liquid membrane (ELM), and biochannel containing supported (immobilised) liquid membrane (SLM), or a combination thereof, wherein said liquid membrane system is based on vesicles formed from amphiphilic compounds such as lipids forming a bilayer wherein biochannels have been incorporated and wherein said vesicles further contain a stabilising oil phase. The uses of the membrane system include water extraction from liquid aqueous media by forward osmosis, e.g. for desalination of salt water.

Claims

1. A liquid membrane system in the form of a biochannel containing bulk liquid membrane (BLM), biochannel containing emulsion liquid membrane (ELM), and biochannel containing supported (immobilised) liquid membrane (SLM), or a combination thereof, wherein the liquid membrane system comprises vesicles formed from one or more amphiphilic compounds forming a bilayer into which the biochannels have been incorporated and wherein the vesicles further comprise a stabilising oil phase.

2. The liquid membrane system according to claim 1, wherein said biochannel is an aquaporin water channel.

3. The liquid membrane system according to claim 1, wherein the biochannel is selected from the group consisting of boron nitride nanotubes, carbon nanotubes, amphiphilic pore forming molecules including the transmembrane channel molecules beta-barrel pores such as alpha-hemolysin and OmpG, FomA, and VDAC;

the transmembrane peptide pores alamethicin, valinomycin, and gramicidin A including derivatives thereof and synthetic peptides;

ion channels, or ion-selective ionophores.

4. The liquid membrane system according to any one of the preceding claims, wherein the amphiphilic compounds are lipids.

5. The liquid membrane system according to any one of the preceding claims, wherein the oil phase comprises components selected from the group consisting of a sterol, squalene, squalane, alpha-tocopherol, hopanoids, isoprenes including esterified dolichol, ubiquinone (Q10), jojoba oil, light mineral oils, linseed oil, soybean oil, ground nut oil, phospholipid stabilized squalene or an emulsion of soy bean oil, phospholipids and glycerin, and alkanes, such as decane, undedane, dodecane;

and mixtures thereof.

6. The liquid membrane system according to any one of the preceding claims, wherein the biochannels are present in a ratio of 1% to about 70% relative to the vesicle surface area.

7. The liquid membrane system according to any one of the preceding claims, wherein the vesicles have an approximate maximal diameter of up to 1000 μ

m.

8. A method of extracting water from an aqueous liquid comprising the following steps:

a) mixing an amount of the liquid membrane system of any one of the preceding claims into a first aqueous liquid having an osmotic pressure which is less than that of the vesicles to form a suspension,

b) allowing the vesicles in said suspension to absorb pure water from said first liquid and expand as long as an osmotic pressure gradient exists,

c) separating the expanded vesicles the from the first liquid, and

d) resuspending said vesicles from step c) in a second aqueous liquid having an osmotic pressure that exceeds the pressure of the expanded vesicles to allow for the extracted water in the vesicles to flow into and dilute said second liquid as long as an osmotic gradient is present leaving the vesicles in a non-expanded state.

9. The method according to claim 7, wherein said first aqueous liquid is selected from the group consisting of a natural water source, such as sea water, river water, lake water, brackish water, rain water;

waste water which is not toxic to the aquaporin water channels;

or biological fluids including wine, fruit and vegetable juice, milk, whole blood, plasma, urine, saliva, sweat or homogenized tissue.

10. The method according to claim 7 or claim 8, wherein the step of separating the expanded vesicles the from the first liquid is carried out by centrifugation or filtration.

11. The method of any one of claims 8 to 10, wherein the method is used for desalination of seawater, wherein salt water is the feed or first aqueous liquid and a CO₂/NH₃ containing aqueous solution is the draw solution or second aqueous liquid.

12. An apparatus for pure water extraction from an aqueous liquid media which comprises one or more liquid membranes according to any one of claims 1 to 7.

13. The apparatus according to claim 12, wherein the apparatus is a two module hollow fiber supported liquid membrane contactor module or a liquid cell extra-flow membrane contactor.

14. A solventless giant protein vesicle consisting essentially of an amphiphilic lipid, transmembrane protein channels, and an oil in an aqueous dispersion, wherein the lipid to protein molar ratio is in the range of from about 1:

50 to about 1;

400.

15. A composition comprising the giant protein vesicles of claim 14.

16. A methods of preparing the giant protein vesicles consisting essentially of an amphiphilic lipid, transmembrane protein channels, and an oil in an aqueous dispersion, and wherein the lipid to protein molar ratio is in the range of from about 1:

50 to about 1;

400, the method comprising the steps of;

a) preparing liposomes from a dried lipid solution that has been rehydrated in a detergent containing buffer and extruded through a filter of about 100 nm to about 500 nm pore size,

b) mixing the liposomes from a) with a transmembrane protein solution, wherein the protein is optionally linked to a fluorescent label,

c) dialysing the mixture from b) overnight using a molecular weight cut off of about 10 kDa,

d) separating the proteoliposome vesicles formed in step c), e.g. by centrifugation,

e) optionally obtaining an absorbance spectrum of the GPVs formed which is compatible with the fluorescent label used in order to verify correct insertion of the transmembrane protein in the vesicle membranes, and

f) mixing the proteoliposomes obtained in step d) with a lipid in an oil phase solution containing the same lipid as in step a) in a molar ratio ranging from about 1;

3 v/v to about 1;

12 v/v,

thereby resulting in the formation of GPVs from the proteoliposomes.

18. The method according to claim 16 or claim 17, wherein the lipid is asolectin.

19. The method according to claim 16 or claim 17, wherein the lipid is DOPC or DPhPC or DOPS or natural lipid extracts, such as E. coli total lipid extract, or soybean mixed phospholipids., or combination mixtures thereof.

20. The method according to any one of claims 16 to 19, wherein said transmembrane protein is selected from aquaporins, such as SoPIP2;

1 and AqpZ; and

transmembrane proteins, such as FomA.

21. The method according to any one of claims 16 to 20, wherein the transmembrane protein is linked to a fluorescent label.

22. The method according to claim 21, wherein the fluorescent label is a naphthalene derivative such as those listed in table 1 herein or a fluorescently functional derivative thereof.

23. The method according to claim 22, wherein the naphthalene derivative is 6-bromoacetyl-2-dimethylaminonaphthalene.

24. Use of the giant protein vesicles prepared according to the method of any one of claims 16 to 23 for extraction of water through forward osmosis.

17. A method of preparing giant protein vesicles consisting essentially of an amphiphilic lipid, transmembrane protein channels, and an oil in an aqueous dispersion, and wherein the lipid to protein molar ratio is in the range of from about 1:

50 to about 1;

400, the method comprising self assembling the vesicles from an aqueous mixture of said amphiphilic lipid, transmembrane protein channels, and oil following end-over-end mixing.

25. A composite filter membrane or disk created by sandwiching a layer of aquaporin containing proteoliposomes or giant protein vesicles in between filter materials selected from ultrafiltration membranes, nanofiltration membranes and microfiltration membranes.

26. A supported liquid membrane having an open or closed sandwich construction, wherein a substantially flat porous filter material provides support on one or both sides of a layer of proteoliposomes, thereby immobilising the layer.