Microbial Oil Production From Biomass Hydrolysate By Oleaginous Yeast Strains
First Claim
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1. A method of producing lipids, comprising the steps of hydrolyzing biomass with dilute acid to form a hydrolysate;
- culturing at least one species of oleaginous yeast in said hydrolysate under conditions that allow said oleaginous yeast to produce and accumulate lipids; and
extracting said lipids from said oleaginous yeast.
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Abstract
Oleaginous yeast strains are used to hydrolyze biomass (e.g. wheat straw) that has been pretreated using dilute acid, in order to produce lipids. The lipids may be used as feedstock for producing biofuels.
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15 Claims
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1. A method of producing lipids, comprising the steps of hydrolyzing biomass with dilute acid to form a hydrolysate;
- culturing at least one species of oleaginous yeast in said hydrolysate under conditions that allow said oleaginous yeast to produce and accumulate lipids; and
extracting said lipids from said oleaginous yeast. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- culturing at least one species of oleaginous yeast in said hydrolysate under conditions that allow said oleaginous yeast to produce and accumulate lipids; and
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9. A method of producing biofuel, comprising the steps of hydrolyzing biomass with dilute acid to form a hydrolysate;
- culturing at least one species of oleaginous yeast in said hydrolysate under conditions that allow said oleaginous yeast to produce and accumulate lipids;
extracting said lipids from said oleaginous yeast; and
producing biofuel from said lipids. - View Dependent Claims (10, 11, 12, 13, 14, 15)
- culturing at least one species of oleaginous yeast in said hydrolysate under conditions that allow said oleaginous yeast to produce and accumulate lipids;
Specification