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THE USE OF CLASS IIB RESTRICTION ENDONUCLEASES IN 2ND GENERATION SEQUENCING APPLICATIONS

  • US 20120094847A1
  • Filed: 05/05/2010
  • Published: 04/19/2012
  • Est. Priority Date: 05/05/2009
  • Status: Active Grant
First Claim
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1. A method for genotyping DNA molecules contained in at least one DNA sample comprising:

  • a) digesting the DNA molecules contained in at least one DNA sample with a class IIB restriction endonuclease to generate DNA fragments;

    b) optionally separating DNA fragments comprising the recognition site for said class IIB restriction endonuclease from the remaining DNA fragments;

    c) attaching at least one adaptor DNA to the 5′ and

    /or 3′

    end of one or both strands of the DNA fragments comprising the recognition site for said class IIB restriction endonuclease obtained in a) or separated in b) to form adaptor-fragment constructs;

    d) determining the sequence of at least a fraction of the DNA fragments obtained in c); and

    e) assigning genotypes to said at least one DNA sample analysed based on the sequence data obtained in d).

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