miRNA BIOMARKERS FOR THE DIAGNOSIS OF DUCHENNE MUSCULAR DYSTROPHY, ITS PROGRESSION AND FOR MONITORING THERAPEUTIC INTERVENTIONS

• US 20120115150A1
• Filed: 05/24/2010
• Published: 05/10/2012
• Est. Priority Date: 05/25/2009
• Status: Abandoned Application

• Current Assignee: Universita' Degli Studi Di Roma La Sapienza (Repubblica Italiana)
• Original Assignee: Universita' Degli Studi Di Roma La Sapienza (Repubblica Italiana)
• Inventors: Bozzoni, Irene, Martone, Julie, Cacchiarelli, Davide, Girardi, Erika
• Application Number:
  US13/322,026
• Publication Number:
  US 20120115150A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: C12Q 2600/178 miRNA, siRNA or ncRNA

Abstract

The invention refers to diagnosis and therapy of muscle degenerative disorders, as Duchenne Muscular Dystrophy (DMD) by means of a class of specific miRNAs.

Claims

1. A method for the diagnosis of a muscle disorder in which muscle fiber degeneration occurs in a subject comprising in vitro determining whether at least one molecule selected from the group consisting of:

miR-206, miR-1, and miR-133 has increased levels in a blood or serum sample of the subject when compared to a sample obtained from a healthy individual.

3. The method according to claim 1 wherein said muscle disorder is Duchenne Muscular Dystrophy.

4. The method according to claim 1 wherein the molecules are miR-206 and miR-1.

5. The method according to claim 1 wherein the detecting of the at least one of molecule is performed by reverse amplification of said molecule and real time detection of amplified products.

2. A method for monitoring the progress of a therapeutic treatment of a muscle disorder in which muscle fiber degeneration occurs in an affected subject comprising in vitro detecting at least one molecule selected from the group consisting of:

miR-206, miR-1, and miR-133 in a blood or serum sample of the subject.