Elite Event A5547-127 and Methods and Kits for Identifying Such Event in Biological Samples
First Claim
Patent Images
1. A method for identifying elite event A5547-127 in biological samples, which method comprises detection of a A5547-127 specific region with a specific primer or probe which specifically recognizes the 5′
- or 3′
flanking region of A5547-127.
2 Assignments
0 Petitions
Accused Products
Abstract
Tools are provided which allow rapid and unequivocal identification of elite event A5547-127 in biological samples.
-
Citations
29 Claims
-
1. A method for identifying elite event A5547-127 in biological samples, which method comprises detection of a A5547-127 specific region with a specific primer or probe which specifically recognizes the 5′
- or 3′
flanking region of A5547-127.
- or 3′
-
2. (canceled)
-
3. (canceled)
-
4. (canceled)
-
5. (canceled)
-
6. (canceled)
-
7. A kit for identifying elite event A5547-127 in biological samples, said kit comprising one primer recognizing the 5′
- flanking region of A5547-127, said 5′
flanking region having the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311 or one primer recognizing the 3′
flanking region of A5547-127, said 3′
flanking region having the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, and one primer recognizing a sequence within the foreign DNA, said foreign DNA having the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 312 to nucleotide 810 or the nucleotide sequence of SEQ ID No 2 from nucleotide 510 to nucleotide 1880. - View Dependent Claims (8, 9, 10)
- flanking region of A5547-127, said 5′
-
11. A primer for use in a A5547-127 PCR identification protocol, having a sequence which, under optimized PCR conditions specifically recognizes a sequence within the 5′
- or 3′
flanking region of A5547-127, said 5′
flanking region having the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311 and said 3′
flanking region having the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 510 to nucleotide 1880. - View Dependent Claims (12, 13)
- or 3′
-
14. A primer comprising at its extreme 3′
- end the sequence of SEQ ID No. 13.
-
15. A primer comprising at its extreme 3′
- end the sequence of SEQ ID No. 15.
-
16. (canceled)
-
17. (canceled)
-
18. (canceled)
- 19. A kit for identifying elite event A5547-127 in biological samples, said kit comprising a specific probe, capable of hybridizing specifically to a specific region of A5547-127.
- 22. A specific probe for the identification of elite event A5547-127 in biological samples.
-
25. A specific probe for the identification of elite event A5547-127 in biological samples, the sequence of being essentially similar to SEQ ID No. 1 from nucleotide 260 to 360 or SEQ ID No. 2 from nucleotide 460 to 560, or the complement of said sequences.
-
26. (canceled)
-
27. (canceled)
-
28. A soybean plant, or cells, parts, seed or progeny thereof, comprising elite event A5547-127 in its genome.
-
29. A DNA
(a) comprising a sequence of between 50 and 500 bp with at least 80% sequence identity to a DNA comprising the 5′ - flanking region of the A5547-127 event and the foreign DNA contiguous therewith, said 5′
flanking region having the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311, and said foreign DNA contiguous therewith comprising the nucleotide sequence of SEQ ID No. 1 from nucleotide 312 to nucleotide 810;(b) comprising a sequence of between 50 and 500 bp with at least 80% sequence identity to a DNA comprising the 3′
flanking region of the A5547-127 event and the foreign DNA contiguous therewith, said 3′
flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, and said the foreign DNA contiguous therewith comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509;
or(c) specifically identifying soybean elite event A5547-127, said DNA comprises the nucleotide sequence of a discriminating amplicon obtainable from a nucleic acid sample comprising said elite event using a polymerase chain reaction with a primer specifically recognizing a sequence within the 5′
or 3′
flanking region of the elite event and a primer specifically recognizing the DNA contiguous therewith, wherein said 5′
flanking region comprises the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311, the foreign DNA contiguous therewith comprises the nucleotide sequence of SEQ ID No. 1 from nucleotide 312 to nucleotide 810, and wherein said 3′
flanking region of A5547-127 comprises the nucleotide sequence of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, and the foreign DNA contiguous therewith comprises the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509.
- flanking region of the A5547-127 event and the foreign DNA contiguous therewith, said 5′
Specification