ELECTRICAL SENSOR FOR ULTRASENSITIVE NUCLEIC ACID DETECTION
First Claim
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1. A sensor for detecting a nucleic acid molecule comprising:
- an electrode arrangement comprising a first electrode, a second electrode and an overlapping region, wherein, within the overlapping region;
a part of the second electrode overlaps a part of the first electrode such that a top surface level of the second electrode is higher than a top surface level of the first electrode;
an insulating layer is provided between the first electrode and the second electrode and is contacting the first electrode and second electrode;
a first nucleic acid probe immobilized at the surface of the first electrode; and
a second nucleic acid probe immobilized at the surface of the second electrode.
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Abstract
The present invention is direct to a sensor for detecting a nucleic acid molecule comprising an electrode arrangement with two electrodes and nucleic acid probes immobilized at the surface of the electrodes. The present invention also refers to a kit and a method of using the sensor or a sensor array. The present invention is further directed to a process of manufacturing a sensor and sensor array.
26 Citations
44 Claims
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1. A sensor for detecting a nucleic acid molecule comprising:
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an electrode arrangement comprising a first electrode, a second electrode and an overlapping region, wherein, within the overlapping region; a part of the second electrode overlaps a part of the first electrode such that a top surface level of the second electrode is higher than a top surface level of the first electrode; an insulating layer is provided between the first electrode and the second electrode and is contacting the first electrode and second electrode; a first nucleic acid probe immobilized at the surface of the first electrode; and a second nucleic acid probe immobilized at the surface of the second electrode. - View Dependent Claims (2, 3, 6, 7, 12, 20, 24, 25, 27, 28, 29, 31, 32, 33, 41, 42)
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4-5. -5. (canceled)
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8-11. -11. (canceled)
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13-19. -19. (canceled)
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21-23. -23. (canceled)
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26. (canceled)
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30. A nucleic acid detection kit comprising:
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a sensor for detecting a nucleic acid molecule comprising; an electrode arrangement comprising a first electrode, a second electrode and an overlapping region, wherein, within the overlapping region; a part of the second electrode overlaps a part of the first electrode such that a top surface level of the second electrode is higher than a top surface level of the first electrode; an insulating layer is provided between the first electrode and the second electrode and is contacting the first electrode and second electrode; a first nucleic acid probe immobilized at the surface of the first electrode; and a second nucleic acid probe immobilized at the surface of the second electrode; a solution comprising a metal precursor; and a solution suitable for chemically reducing the metal precursor.
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34-40. -40. (canceled)
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43. A method of detecting a target nucleic acid comprising:
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providing a sensor for detecting a nucleic acid molecule comprising; an electrode arrangement comprising a first electrode, a second electrode and an overlapping region, wherein, within the overlapping region; a part of the second electrode overlaps a part of the first electrode such that a top surface level of the second electrode is higher than a top surface level of the first electrode; an insulating layer is provided between the first electrode and the second electrode and is contacting the first electrode and second electrode; a first nucleic acid probe immobilized at the surface of the first electrode; and a second nucleic acid probe immobilized at the surface of the second electrode, wherein the sensor comprises two electrodes and wherein nucleic acid sequences are immobilized at the surface of the electrodes which are complementary to the target nucleic acid sequence; incubating the sensor with a sample fluid suspected to comprise the target nucleic acid; metallizing the nucleic acid molecules of the sensor; and carrying out conductance measurements to determine the presence or absence of the target nucleic acid. - View Dependent Claims (44)
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Specification