Analysis Chip and Analysis Apparatus

• US 20120125773A1
• Filed: 02/01/2012
• Published: 05/24/2012
• Est. Priority Date: 04/27/2007
• Status: Abandoned Application

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Nakayama, Yusuke, Sugiyama, Koji
• Application Number:
  US13/364,129
• Publication Number:
  US 20120125773A1
• Time in Patent Office: Days
• US Class Current: 204/451
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

An analysis chip that enables an apparatus to be small, analysis to be simple, analysis time to be short and analysis of both glycosylated hemoglobin and glucose to be highly accurate is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, a first introduction reservoir 2a, a first recovery reservoir 2b and a capillary channel for sample analysis 3x; the first introduction reservoir 2a and the first recovery reservoir 2b are formed in the lower substrate 1; and the first introduction reservoir 2a and the first recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x.

Claims

1. A method of analyzing glycosylated hemoglobin and glucose using an analysis chip, wherein the glycosylated hemoglobin is analyzed by a capillary electrophoresis method and the glucose is analyzed by a color developing method, the analysis chip comprising:

a substrate, a plurality of fluid reservoirs and a capillary channel for the capillary electrophoresis method,

the plurality of fluid reservoirs comprising a first introduction reservoir and a first recovery reservoir,

the capillary channel comprising a capillary channel for sample analysis,

the first introduction reservoir and the first recovery reservoir being formed in the substrate, and

the first introduction reservoir and the first recovery reservoir being in communication with each other via the capillary channel for sample analysis,

the glucose analysis reagent including a reagent that develops a color in association with a redox reaction that uses glucose as a substrate and being disposed in at least one reservoir selected from the group consisting of the plurality of fluid reservoirs and a reservoir other than the plurality of fluid reservoirs formed in the substrate, and

the glucose being analyzed according to a color developing method using the glucose analysis reagent and an optical measurement instrument.

2. The method according to claim 1, wherein in the analysis chip

the plurality of fluid reservoirs further comprises a second introduction reservoir and a second recovery reservoir,

the capillary channel further comprises a capillary channel for sample introduction,

the second introduction reservoir and the second recovery reservoir are formed in the substrate,

the second introduction reservoir and the second recovery reservoir are in communication with each other via the capillary channel for sample introduction,

the capillary channel for sample analysis and the capillary channel for sample introduction intersect, and

the capillary channel for sample analysis and the capillary channel for sample introduction are in communication with each other at the intersection.

3. The method according to claim 2, wherein in the analysis chip

a first branching channel branches off from a part of the capillary channel for sample analysis,

the first branching channel is in communication with the second introduction reservoir,

a second branching channel branches off from a part of the capillary channel for sample analysis that is located on the downstream side relative to the first branching channel,

the second branching channel is in communication with the second recovery reservoir, and

the capillary channel for sample introduction is formed by the first branching channel, the second branching channel, and a part of the capillary channel for sample analysis that connects the branching channels.

4. The method according to claim 1, wherein the analysis chip has a maximum length of the whole chip in a range of 10 to 100 mm, a maximum width of the whole chip in a range of 10 to 60 mm, and a maximum thickness of the whole chip in a range of 0.3 to 5 mm.

5. The method according to claim 1, wherein, in analyzing glycosylated hemoglobin and glucose, a diluted sample prepared by diluting a sample containing a glycosylated hemoglobin and glucose with an electrophoresis running buffer is introduced into at least one reservoir among the plurality of fluid reservoirs, and a volume ratio of the sample:

the electrophoresis running buffer is 1;

4 to 1;

99.

6. The method according to claim 1, wherein the capillary channel is filled with an electrophoresis running buffer.

7. The method according to claim 1, wherein the capillary channel has a maximum diameter in a range of 10 to 200 μ

m and a maximum length of 0.5 to 15 cm.

8. The method according to claim 1, wherein

the reservoir other than the plurality fluid reservoirs formed in the substrate comprises a pretreatment reservoir,

the pretreatment reservoir and the plurality of fluid reservoirs are in communication with each other, and wherein

in the pretreatment reservoir

a sample containing glycosylated hemoglobin and glucose is hemolyzed and diluted.

10. The method according to claim 8, wherein,

the reservoir other than the plurality of fluid reservoirs formed in the substrate comprises a reagent reservoir, and

the reagent reservoir is in communication with at least one reservoir among the plurality of reservoirs and the pretreatment reservoir.

17. The method according to claim 1, wherein the glycosylated hemoglobin is HbA1c.

18. The method according to claim 1, wherein in the analysis chip

the substrate comprises an upper substrate and a lower substrate,

a plurality of through-holes are formed in the upper substrate,

a groove is formed in the lower substrate,

the upper substrate is laminated onto the lower substrate,

spaces created by sealing the bottom parts of the plurality of through-holes formed in the upper substrate with the lower substrate serve as the plurality of fluid reservoirs, and

a space created by sealing the upper part of the groove formed in the lower substrate with the upper substrate serves as the capillary channel.

19. The method according to claim 1, wherein in the analysis chip

a plurality of concave portions and a groove are formed in the substrate,

a surface of the substrate is sealed with a sealing material that has openings at places corresponding to the plurality of concave portions,

the plurality of concave portions formed in the substrate serve as the plurality of fluid reservoirs, and

a space created by sealing the upper part of the groove formed in the substrate with the sealing material serves as the capillary channel.

20. The method according to claim 1, wherein

the analysis chip further includes a sealing material, and wherein in the analysis chip

a plurality of through-holes are formed in the substrate,

a groove is formed in the bottom surface of the substrate,

the bottom surface of the substrate is sealed with the sealing material,

spaces created by sealing the bottom parts of the plurality of through-holes formed in the substrate with the sealing material serve as the plurality of fluid reservoirs, and

a space created by sealing the lower part of the groove formed in the bottom surface of the substrate with the sealing material serves as the capillary channel.

21. The method according to claim 1, wherein in the analysis chip

the plurality of fluid reservoirs are in communication with each other via a capillary tube that is a member independent of the substrate, and the capillary tube serves as the capillary channel.

22. The method according to claim 1, wherein in the analysis chip

the plurality of fluid reservoirs each has a volume in a range of 1 to 1000 mm³.

23. The method according to claim 1, wherein

the analysis chip further comprises a plurality of electrodes for use with a capillary electrophoresis method,

wherein the plurality of electrodes for use with a capillary electrophoresis method are disposed such that their one ends are disposed respectably in the plurality of fluid reservoirs.

9. (canceled)

11-16. -16. (canceled)

24-25. -25. (canceled)