USE OF MICROFLUIDIC SYSTEMS IN THE DETECTION OF TARGET ANALYTES USING MICROSPHERE ARRAYS
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Abstract
The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
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Citations
47 Claims
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1-18. -18. (canceled)
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19. A method of sequencing a nucleic acid, said method comprising:
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obtaining a fluid comprising a nucleic acid; obtaining a substrate comprising a primer, said primer in fluid communication with a flow channel having an inlet port; providing said fluid to said flow channel, wherein said electroosmotic pump is in fluid communication with the inlet port, and wherein said fluid is distributed through the flow channel by the electroosmotic pump; allowing the nucleic acid to bind to said primer; amplifying said nucleic acid, thereby generating an amplified target nucleic acid; and sequencing said amplified target nucleic acid. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A microfluidic system for detecting a target analyte comprising:
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a flow channel system for delivering fluid to a detection module comprising a flow channel and a fluid pump configured to deliver fluid to the flow channel; a detection module for detecting a target analyte comprising a substrate having wells thereon, a population of beads comprising a first bead comprising a first binding ligand, and a second bead comprising a second binding ligand, the first and second beads randomly distributed in at least one of the wells; a control system for controlling the flow channel system or detection module. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification