INDUCED DERIVATION OF SPECIFIC ENDODERM FROM hPS CELL-DERIVED DEFINITIVE ENDODERM
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Abstract
The present invention relates to a method to control differentiation of human pluripotent stem cells, including human balstocyst derived stem (hBS) cells and to obtain specific endoderm cells. In particular, present invention relates to the use of FGF2 as the key factor in a specific concentration to control differentiation of definitive endoderm cells derived from hPS cells to specific endoderm cells. The invention also provides methods of obtaining endoderm cells comprising the use of FGFR and activation of the MAPK signalling pathway.
9 Citations
52 Claims
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1-33. -33. (canceled)
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34. A method to control differentiation of definitive endodermal cells derived from human pluripotent stem (hPS) cells comprising:
providing a concentration of fibroblast growth factor 2 (FGF2) to control differentiation of definitive endodermal cells derived from hPS cells to specific endoderm cells. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 52)
- 45. A method for the preparation of pancreatic endodermal cells, the method comprising incubating definitive endodermal cells in a culture medium comprising from about 16 ng/ml to about 150 ng/ml FGF2 for about 2 to about 20 days.
Specification