Inoculants Including Bacillus Bacteria for Inducing Production of Volatile Organic Compounds in Plants

• US 20120149571A1
• Filed: 12/12/2011
• Published: 06/14/2012
• Est. Priority Date: 12/10/2010
• Status: Active Grant

• Current Assignee: Auburn University
• Original Assignee: Auburn University
• Inventors: Fadamiro, Henry Y., Kloepper, Joseph W., Ngumbi, Esther N., Nangle, Kate W.
• Granted Patent: US 8,445,255 B2
• Time in Patent Office: Days
• US Class Current: 504/117
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 63/22 Bacillus
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/07 Bacillus

Abstract

Disclosed are inoculants that include Bacillus bacteria and induce production of volatile organic compounds (VOCs) by a plant that has been treated with the inoculant.

Claims

1. An inoculant for a plant comprising isolated Bacillus bacteria and a carrier, the inoculant inducing production of one or more volatile organic compounds (VOCs) by a plant that has been treated with the inoculant.

2. The inoculant of claim 1, wherein the bacteria are selected from a group consisting of Bacillus amyloliquefaciens, Bacillus mojavensis, Bacillus solisalsi, Bacillus pumilus, Bacillus simplex, Bacillus subtilis and mixtures thereof.

3. The inoculant according to claim 1, wherein the bacteria are plant growth-producing rhizobacteria (PGPR).

4. The inoculant according to claim 1, wherein the one or more VOCs comprise one or more compounds selected from a group consisting of alpha-pinene, beta-pinene, beta-myrcene, cis-3-hexenyl acetate, limonene, beta-ocimene, linalool, (E)-4,8-dimethyl-1,3,7-nonatriene, methyl salicylate, decanal, cis-jasmone, caryophyllene, alpha-humulene, beta-farnesene, and mixtures thereof.

5. The inoculant according to claim 1, wherein the insect is an herbivore and the one or more VOCs reduce egg-laying of the insect on the plant.

6. The inoculant according to claim 1, wherein the insect is an herbivore and the one or more VOCs reduce feeding of the insect on the plant.

7. The inoculant according to claim 1, wherein the insect is a predator or a parasitoid and the one or more VOCs attract the predator or the parasitoid to the plant.

8. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence comprising SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, or SEQ ID NO;

6.

9. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 98% identical to SEQ ID NO:

1.

10. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 98% identical to SEQ ID NO:

2.

11. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 91% identical to SEQ ID NO:

3.

12. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 96% identical to SEQ ID NO:

4.

13. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 93% identical to SEQ ID NO:

5.

14. The inoculant according to claim 1, wherein the bacteria have a 16S rDNA nucleic acid sequence that is at least 93% identical to SEQ ID NO:

6.

15. The inoculant according to claim 1, wherein the bacteria are Bacillus amyloliquefaciens or a mixture comprising Bacillus amyloliquefaciens.

16. The inoculant according to claim 15, wherein the bacteria are selected from a group consisting of Bacillus amyloliquefaciens strain AP-136, Bacillus amyloliquefaciens strain AP-188 Bacillus amyloliquefaciens strain AP-218, Bacillus amyloliquefaciens strain AP-219, and Bacillus amyloliquefaciens strain AP-295.

17. The inoculant according to claim 1, wherein the bacteria are Bacillus mojavensis or a mixture comprising Bacillus mojavensis.

18. The inoculant according to claim 17, wherein the bacteria are Bacillus mojavensis strain AP-209.

19. The inoculant according to claim 1, wherein the bacteria are Bacillus solisalsi or a mixture comprising Bacillus solisalsi.

20. The inoculant according to claim 19, wherein the bacteria are Bacillus solisalsi strain AP-217.

21. The inoculant according to claim 1, wherein the bacteria are Bacillus pumilus or a mixture comprising Bacillus pumilus.

22. The inoculant according to claim 21, wherein the bacteria are Bacillus pumilus strain INR-7.

23. The inoculant according to claim 1, wherein the bacteria are Bacillus simplex or a mixture comprising Bacillus simplex.

24. The inoculant according to claim 1, wherein the bacteria are Bacillus simplex strain ABU 288.

25. The inoculant according to claim 1, wherein the bacteria are Bacillus subtilis strain MBI 600.

26. A method of modifying insect behavior towards a plant, the method comprising a administering the inoculant of claim 1 to the plant, to seeds of the plant, or to soil surrounding the plant.

27. The method according to claim 26, wherein the insect is an herbivore and the method reduces egg-laying of the insect on the plant.

28. The method according to claim 26, wherein the insect is an herbivore and the method reduces feeding of the insect on the plant.

29. The method according to claim 26, wherein the insect is a predator or a parasitoid and the method attracts the predator or parasitoid to the plant.

30. The method according to claim 26, wherein the plant is selected from a group consisting of alfalfa, rice, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, lentil chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, soybean, canola, oil seed rape, spring wheat, winter wheat, tobacco, tomato, sorghum, and sugarcane.