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BINDING-INDUCED HAIRPIN DETECTION SYSTEM

  • US 20120156678A1
  • Filed: 08/30/2010
  • Published: 06/21/2012
  • Est. Priority Date: 08/31/2009
  • Status: Active Grant
First Claim
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1. A binding-induced hairpin detection system comprising:

  • a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and

    b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe;

    wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.

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