CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS
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Abstract
The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.
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Citations
33 Claims
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1-24. -24. (canceled)
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25. A method for characterising a nucleic acid polymer, the method comprising:
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a. providing two separate pools of solution and an impermeable barrier between the two pools, the barrier containing an ion-permeable protein pore; b. placing the nucleic acid polymer in one of the two pools; c. measuring current flow across the ion-permeable protein pore in the presence of a voltage differential between the two pools; and d. determining sequence-dependent characteristics of the nucleic acid polymer. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33)
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Specification