RSV F PROTEIN COMPOSITIONS AMD METHODS FOR MAKING SAME

• US 20120164176A1
• Filed: 07/15/2010
• Published: 06/28/2012
• Est. Priority Date: 07/15/2009
• Status: Abandoned Application

• Current Assignee: Novartis Ag
• Original Assignee: Novartis Ag
• Inventors: Swanson, Kurt, Dormitzer, Philip
• Application Number:
  US13/383,897
• Publication Number:
  US 20120164176A1
• Time in Patent Office: Days
• US Class Current: 424/211.1
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/5256 expressing foreign proteins
• CPC Class Codes: A61K 2039/5258 Virus-like particles
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 2039/55505 Inorganic adjuvants
• CPC Class Codes: A61K 2039/55555 Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
• CPC Class Codes: A61K 2039/55561 CpG containing adjuvants; Oligonucleotide containing adjuvants
• CPC Class Codes: A61K 2039/55566 Emulsions, e.g. Freund's adjuvant, MF59
• CPC Class Codes: A61K 2039/55572 Lipopolysaccharides; Lipid A; Monophosphoryl lipid A
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/155 Paramyxoviridae, e.g. parainfluenza virus
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/1027 Paramyxoviridae, e.g. respiratory syncytial virus
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/21 containing a His-tag
• CPC Class Codes: C07K 2319/50 : containing protease site
• CPC Class Codes: C07K 2319/73 : containing coiled-coiled motif (leucine zippers)
• CPC Class Codes: C12N 2760/18522 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/18534 : Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/36143 : viral genome or elements thereof as genetic vector

Abstract

The present invention relates to immunogenic compositions comprising RSV F protein, methods for preparing compositions that contain RSV F protein ecto-domain polypeptides, and to certain engineered RSV F proteins and nucleic acids that encode the engineered RSV F proteins. Compositions prepared using the methods can contain RSV F protein ecto-domain polypeptides in a predominant or single desired form and conformation. The invention also relates to methods for inducing an immune response to RSV F.

Claims

1-98. -98. (canceled)

99. An immunogenic composition comprising a population of soluble respiratory syncytial virus F (RSV F) polypeptides that are in the form of monomers, trimers, or a combination of monomers and trimers.

100. The immunogenic composition of claim 99, wherein the timers are monodispersed trimers.

101. The immunogenic composition of claim 99, wherein the RSV F polypeptides are in the form of a combination of monomers and trimers.

102. The immunogenic composition of claim 101, wherein the monomers and trimers are in dynamic equilibrium.

103. The immunogenic composition of claim 99, wherein the soluble RSV F polypeptides are ectodomain polypeptides.

104. The immunogenic composition of claim 103, wherein the soluble RSV F polypeptides are uncleaved.

105. The immunogenic composition of claim 103, wherein the soluble RSV F polypeptides are cleaved.

106. The immunogenic composition of claim 99, further comprising an adjuvant.

107. A method of inducing an immune response in a subject to RSV F comprising administering said subject an immunogenic composition of claim 99.