DIGITAL DROPLET SEQUENCING

• US 20120164633A1
• Filed: 12/27/2011
• Published: 06/28/2012
• Est. Priority Date: 12/27/2010
• Status: Abandoned Application

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Laffler, Thomas
• Application Number:
  US13/337,819
• Publication Number:
  US 20120164633A1
• Time in Patent Office: Days
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2563/159 Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Abstract

The present invention provides systems, devices, methods, kits, and compositions for sorting and analysis of nucleic acid sequences using digital droplet PCR. In particular, provided herein are methods to convert complex samples into a plurality of simplified samples, and sequence analysis thereof.

Claims

1. A method of analyzing nucleic acid comprising:

a) separating a nucleic acid sample into a plurality of partitions, wherein said nucleic acid sample comprises a mixture of nucleic acid molecules and amplification reagents, wherein a portion of said plurality of partitions are single nucleic acid molecule containing partitions, and a portion of said plurality of partitions are zero nucleic acid molecule containing partitions, and the number of partitions containing more than one nucleic acid molecule is zero or a statistically insignificant fraction of the total number of partitions;

b) treating said plurality of partitions under amplification conditions such that said single nucleic acid molecule containing partitions become amplicon-containing partitions; and

c) physically sorting said plurality of partitions.

2. The method of claim 1, wherein said sorting comprises physically separating said amplicon-containing partitions from partitions not containing amplicons.

3. The method of claim 1, wherein said sorting comprises physically separating said amplicon-containing partitions from said zero nucleic acid molecule containing partitions.

4. The method of claim 1, wherein said amplification reagents comprise at least one set of primers.

5. The method of claim 4, wherein said amplification reagents comprise two or more sets of primers.

6. The method of claim 5, wherein each set of primers is configured to amplify a different set of target amplicons.

7. The method of claim 6, wherein said different sets of target amplicons are differentially labeled during amplification.

8. The method of claim 7, wherein said sorting comprises physically separating said differentially labeled sets of target amplicons.

9. The method of claim 1, further comprising d) determining the sequence and/or mass of amplicons in said amplicon-containing partitions.

10. The method of claim 1, wherein said nucleic acid sample further comprises detection reagents.

11. The method of claim 10, wherein said detection reagents comprise labels.

12. The method of claim 11, wherein said labels comprise fluorescent labels.

13. The method of claim 11, further comprising a step of labeling the nucleic acid molecules to produce labeled amplicons.

14. The method of claim 1, wherein said sample is selected from an environmental sample, a biological sample, a clinical sample, and a forensic sample.

15. The method of claim 1, wherein said partitions comprise droplets.

16. The method of claim 1, wherein analyzing the sequence or mass of the amplicons comprises determining the nucleotide sequence of all or a portion of the amplicon.

17. The method of claim 1, wherein analyzing the mass of the amplicons is performed by mass spectrometry.

18. The method of claim 1, further comprising the steps of:

d) re-amplifying the amplicons to produce clonal populations of amplicons; and

e) determining the sequence and/or mass of amplicons in said amplicon-containing partitions.