Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
First Claim
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1. An oligonucleotide comprising the primary sequence of oligonucleotides selected from SEQ ID NOs. 2, 10, 18, 27, 32, 51, 60, 71, 82, 93 and 104.
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Accused Products
Abstract
The invention comprises reagents and methods for detecting cancer-causing mutations in the human EGFR gene. Further, a method of detecting the mutations and a method of treatment are disclosed.
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Citations
20 Claims
- 1. An oligonucleotide comprising the primary sequence of oligonucleotides selected from SEQ ID NOs. 2, 10, 18, 27, 32, 51, 60, 71, 82, 93 and 104.
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5. An oligonucleotide selected from SEQ ID NOs. 3-7, 11-15, 19-24, 28, 29, 33-48, 52-57, 61-68, 72-79, 83-90, 94-101, 105 and 106.
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6. An oligonucleotide selected from SEQ ID NOs. 8, 16, 25, 30, 49, 58, 69, 80, 91, 102 and 107.
- 7. An oligonucleotide selected from SEQ ID NOs. 9 and 109, 17 and 110, 26 and 111, 31 and 112, 50 and 113, 59 and 114, 70 and 115, 81 and 116, 92 and 117, 103 and 118 and 108 and 119.
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15. A kit for detecting mutations in the human epidermal growth factor receptor (EGFR) gene comprising one or more pairs of oligonucleotides selected from pairs (a)-(k):
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(a) an oligonucleotide of one of SEQ ID NOs;
2-7 and the oligonucleotide of SEQ ID NO;
8;(b) an oligonucleotide of one of SEQ ID NOs;
10-15 and the oligonucleotide of SEQ ID NO;
16;(c) an oligonucleotide of one of SEQ ID NOs;
18-24 and the oligonucleotide of SEQ ID NO;
25;(d) an oligonucleotide of one of SEQ ID NOs;
27-29 and the oligonucleotide of SEQ ID NO;
30;(e) an oligonucleotide of one of SEQ ID NOs;
32-48 and the oligonucleotide of SEQ ID NO;
49;(f) an oligonucleotide of one of SEQ ID NOs;
51-57 and the oligonucleotide of SEQ ID NO;
58;(g) an oligonucleotide of one of SEQ ID NOs;
60-68 and the oligonucleotide of SEQ ID NO;
69;(h) an oligonucleotide of one of SEQ ID NOs;
71-79 and the oligonucleotide of SEQ ID NO;
80;(i) an oligonucleotide of one of SEQ ID NOs;
82-90 and the oligonucleotide of SEQ ID NO;
91;(j) an oligonucleotide of one of SEQ ID NOs;
93-101 and the oligonucleotide of SEQ ID NO;
102;(k) an oligonucleotide of one of SEQ ID NOs;
104-106 and the oligonucleotide of SEQ ID NO;
107. - View Dependent Claims (16, 17, 18)
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19. A reaction mixture for detecting mutations in the human epidermal growth factor receptor (EGFR) gene comprising one or more pairs of oligonucleotides selected from pairs (a)-(k):
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an oligonucleotide of one of SEQ ID NOs;
2-7 and the oligonucleotide of SEQ ID NO;
8;an oligonucleotide of one of SEQ ID NOs;
10-15 and the oligonucleotide of SEQ ID NO;
16;an oligonucleotide of one of SEQ ID NOs;
18-24 and the oligonucleotide of SEQ ID NO;
25;an oligonucleotide of one of SEQ ID NOs;
27-29 and the oligonucleotide of SEQ ID NO;
30;an oligonucleotide of one of SEQ ID NOs;
32-48 and the oligonucleotide of SEQ ID NO;
49;an oligonucleotide of one of SEQ ID NOs;
51-57 and the oligonucleotide of SEQ ID NO;
58;an oligonucleotide of one of SEQ ID NOs;
60-68 and the oligonucleotide of SEQ ID NO;
69;an oligonucleotide of one of SEQ ID NOs;
71-79 and the oligonucleotide of SEQ ID NO;
80;an oligonucleotide of one of SEQ ID NOs;
82-90 and the oligonucleotide of SEQ ID NO;
91;an oligonucleotide of one of SEQ ID NOs;
93-101 and the oligonucleotide of SEQ ID NO;
102;an oligonucleotide of one of SEQ ID NOs;
104-106 and the oligonucleotide of SEQ ID NO;
107.
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20. The reaction mixture of claim 22, further comprising an additional oligonucleotide as follows:
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the reaction mixture with the pair of oligonucleotides (a) further comprising the oligonucleotide of SEQ ID NOS 9 and 109; the reaction mixture with the pair of oligonucleotides (b) further comprising the oligonucleotide of SEQ ID NOS 17 and 110; the reaction mixture with the pair of oligonucleotides (c) further comprising the oligonucleotide of SEQ ID NOS 26 and 111; the reaction mixture with the pair of oligonucleotides (d) further comprising the oligonucleotide of SEQ ID NOS 31 and 112; the reaction mixture with the pair of oligonucleotides (e) further comprising the oligonucleotide of SEQ ID NOS 50 and 113; the reaction mixture with the pair of oligonucleotides (f) further comprising the oligonucleotide of SEQ ID NOS 59 and 114; the reaction mixture with the pair of oligonucleotides (g) further comprising the oligonucleotide of SEQ ID NOS 70 and 115; the reaction mixture with the pair of oligonucleotides (h) further comprising the oligonucleotide of SEQ ID NOS 81 and 116; the reaction mixture with the pair of oligonucleotides (i) further comprising the oligonucleotide of SEQ ID NOS 92 and 117; the reaction mixture with the pair of oligonucleotides (j) further comprising the oligonucleotide of SEQ ID NOS 103 and 118; the reaction mixture with the pair of oligonucleotides (k) further comprising the oligonucleotide of SEQ ID NOS 108 and 119.
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Specification