COMPOSITIONS AND METHODS FOR IDENTIFYING AND DETECTING SITES OF TRANSLOCATION AND DNA FUSION JUNCTIONS

• US 20120178635A1
• Filed: 08/06/2010
• Published: 07/12/2012
• Est. Priority Date: 08/06/2009
• Status: Abandoned Application

• Current Assignee: University of Virginia
• Original Assignee: University of Virginia Patent Foundation (University of Virginia)
• Inventors: Dutta, Anindya, Shibata, Yoshiyuki, Malhotra, Ankit
• Application Number:
  US13/389,261
• Publication Number:
  US 20120178635A1
• Time in Patent Office: Days
• US Class Current: 506/4
• CPC Class Codes: C12N 15/1065 Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
• CPC Class Codes: C12N 15/1093 General methods of preparing gene libraries, not provided for in other subgroups

Abstract

The present invention provides a powerful technique based on ultra high-throughput sequencing that finds structural aberrations of chromosomes and defines breakpoints. It is disclosed herein that, Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, is a cost-effective means to identify chromosomal aberrations and define breakpoints. Using this method, we defined the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome positive cell lines and patient cells. This DNA-based method is highly sensitive and can detect signal using samples from which it is hard to obtain RNA or cells where the RNA expression has been silenced. These data demonstrate that ChromPET is a cost-effective and powerful technology that can identify and follow the appearance of chromosomal aberrations in various organisms, including, but not limited to, humans.

Claims

1. A method for identifying a structural variation in a chromosome, said method comprising:

a. constructing a ChromPET library from a DNA sample, fragmenting said DNA, and adding a Y-shaped adapter with a bar code to both ends of said fragments wherein paired-end tags are formed;

b. preparing RNA bait;

c. heat denaturing said ChromPET library DNA and hybridizing said ChromPET library DNA to said RNA bait;

d. capturing said RNA-DNA hybrid;

e. washing away said RNA, converting annealed DNA to double-stranded DNA, and sequencing said DNA, forming an Anchored ChromPET library;

f. identifying said ChromPETs from said Anchored ChromPET library using the bar code of the paired-end tags;

g. mapping said ChromPETs from said Anchored ChromPET library to a targeted sequence region, extracting a sequence of interest, and indexing said sequence of interest;

h. classifying said ChromPETs from said Anchored ChromPET library as normal or aberrant ChromPETs, wherein said aberrant ChromPET is optionally a junctional ChromPET;

i. mapping said junctional ChromPETs to the genome; and

j. predicting chromosomal breakpoints, thereby identifying a structural variation in a chromosome.

2. The method of claim 1, wherein said DNA sequencing is high-throughput sequencing.

3. The method of claim 1, wherein said ChromPET library fragments are about 0.5 kb.

4. The method of claim 1, wherein said identified ChromPETs from said Anchored ChromPET library are identified based on paired-end tag reads obtained from a sequencer.

5. The method of claim 4, wherein said identified ChromPETs are mapped to a target region using an alignment program.

6. The method of claim 1, wherein said sequence of interest is indexed with an indexing program.

7. The method of claim 1, wherein said breakpoint is predicted using an algorithm.

8. The method of claim 7, wherein said algorithm is based on a voting procedure.

9. The method of claim 8, wherein each tag of a junctional ChromPET votes on the location of the actual breakpoint and normal ChromPETs are used to estimate average and standard deviation of fragment lengths.

10. The method of claim 9, wherein when each tag of a junctional ChromPET votes on the location of the actual breakpoint, a vote of 3 is to the interval that is the average fragment length downstream from the start of the tag, a vote of 2 is to the interval one standard deviation down from the end of the 3 zone, and a vote of 1 is to the interval another standard deviation downstream from the 2 zone, further wherein all votes are totaled and plotted over a locus of interest, and the region with the maximum votes contains the predicted breakpoint.

11. The method of claim 10, wherein said locus of interest is selected from the group consisting of BCR and ABL.

12. The method of claim 1, wherein the normal ChromPETs are selected from the group consisting of BCR-BCR and ABL1-ABL-1.

13. The method of claim 1, wherein the junctional ChromPETs are associated with a disease or disorder.

18. The method of claim 1, wherein said structural variation is selected from the group consisting of rearrangement, deletion, insertion, translocation, and copy number change.

19. The method of claim 1, wherein said chromosome is a mammalian chromosome.

20. The method of claim 19, wherein the mammal is a human.

21. A method for diagnosing a disease or disorder in a subject, said method comprising identifying a structural variation in a chromosome of a test subject according to the method of claim 1, wherein said structural variation is associated with said disease or disorder, thereby diagnosing a disease or disorder in a subject.

25. A method for monitoring the progression of a disease or disorder in a subject wherein said disease or disorder is associated with a structural variation in a chromosome, said method comprising measuring the level of said structural variation in a sample from a test subject according to the method of claim 1, comparing said level in said test subject to the level of said structural variation in an otherwise identical sample obtained earlier from said test subject or from an unaffected subject, or to a standard sample, thereby monitoring the progression of a disease or disorder in a subject.

30. The method of claim 25, wherein a higher level of said structural variation in said test subject compared to the level in an otherwise identical sample obtained earlier from said test subject or from an unaffected subject, or to a standard sample, is an indication that said disease or disorder is progressing.

31. The method of claim 25, wherein a lower level of said structural variation in said test subject compared to the level in an otherwise identical sample obtained earlier from said test subject or from an unaffected subject, or to a standard sample, is an indication that said disease or disorder is regressing.

14-16. -16. (canceled)

22-24. -24. (canceled)

26-29. -29. (canceled)

32. A kit for identifying and measuring a structural variation in a chromosome, said kit comprising:

a list of reagents for pre-treatment of selected DNA sites for anchoring;

polymerase chain reaction reagents;

primer sequences;

a list of reagents for sequencing;

instructions and reagents for making the DNA library for paired end tag sequencing;

DNA sequence based bar codes to distinguish libraries from different patients;

list of materials for computational platform;

algorithm for searching the ChromPET sequences to identify translocation junctions;

primers to PCR amplify the translocation junction and sequence the junctional fragments for confirmation; and

an instructional material for the use thereof.